Integrin beta 3 Rabbit Polyclonal Antibody
cat.: ER1601-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 87/110 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Integrin beta-3 aa 481-530 / 788. |
| Positive control: | U-87 MG, HUVEC, Mouse spleen tissue lysate, rat ossature tissue, human tonsil tissue, human kidney tissue, human placenta tissue, mouse kidney tissue, HUVEC. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: P05106 Human | O54890 Mouse |
| Alternative names: | Antigen identified by monoclonal L230 CD 51 CD 61 CD51 CD51 antigen CD61 CD61 antigen DKFZp686A08142 GP3A GPIIIa Integrin alpha V Integrin alpha V vitronectin receptor alpha polypeptide Integrin alpha V vitronectin receptor alpha polypeptide antigen CD51 integrin alpha-V Integrin alpha-V light chain Integrin beta 3 Integrin beta 3 platelet glycoprotein IIIa antigen CD61 Integrin beta chain beta 3 integrinVbeta3 ITAV_HUMAN ITGAV ITGB 3 ITGB3 MSK 8 MSK8 Platelet glycoprotein IIIa Platelet glycoprotein IIIa precursor Platelet membrane glycoprotein IIIa Vitronectin receptor alpha polypeptide vitronectin receptor Vitronectin receptor subunit alpha VNR 1 VNR1 VNRA |
Images
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Fig1:
Western blot analysis of Integrin beta 3 on different lysates with Rabbit anti-Integrin beta 3 antibody (ER1601-4) at 1/1,000 dilution. Lane 1: U-87 MG (Human glioblastoma cell) cell lysate 20 µg/Lane Lane 2: HUVEC (Human umbilical vein endothelial cell) cell lysate 20 µg/Lane Lane 3: Mouse spleen tissue lysate 40 µg/Lane Exposure time: 25 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1601-4, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 87.1 kDa Observed band size: 97 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat ossature tissue using anti-Integrin beta-3 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Integrin beta-3 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Integrin beta-3 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Integrin beta-3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Integrin beta-3 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.