NM23 Rabbit Polyclonal Antibody
cat.: ER1603-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IHC-P, FC, WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 17/19 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human USP36 aa 64-128. |
| Positive control: | Hela, HepG2, MCF-7, human lung cancer tissue, human breast cancer tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
IF-Cell IHC-P FC WB |
1:50-1:200 1:50-1:200 1:50-1:100 1:500 |
| Uniprot #: | SwissProt: P15531 Human | P15532 Mouse |
| Alternative names: | AWD AWD, drosophila, homolog of GAAD Granzyme A activated DNase Granzyme A-activated DNase GZMA activated DNase Metastasis inhibition factor NM23 NB NBS NDK A NDKA NDKA_HUMAN NDP kinase A NDPK-A NDPKA NM23 NM23 long variant, included nm23-H1 NM23-M1 NM23H1B, included NME/NM23 nucleoside diphosphate kinase 1 Nme1 NME1-NME2 spliced read-through transcript, included Non-metastatic cells 1, protein (NM23A) expressed in Nonmetastatic cells 1, protein expressed in Nonmetastatic protein 23 Nonmetastatic protein 23, homolog 1 Nucleoside diphosphate kinase A Tumor metastatic process-associated protein |
Images
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Fig1: ICC staining NM23 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining NM23 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining NM23 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-NM23 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NM23 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-NM23 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Jurkat cells with NM23 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.