SNAIL Rabbit Polyclonal Antibody
cat.: ER1706-22
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SNAIL aa 1-200. |
| Positive control: | A549 cell lysate, 293T cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, NIH/3T3. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell |
1:5,000 1:50 |
| Uniprot #: | SwissProt: O95863 Human | Q02085 Mouse Entrez Gene: 116490 Rat |
| Alternative names: | dJ710H13.1 Protein sna Protein snail homolog 1 Protein snail homolog SLUGH2 SNA Sna protein SNAH SNAI snai1 SNAI1_HUMAN Snail 1 homolog Snail 1 zinc finger protein SNAIL Snail family transcriptional repressor 1 Snail homolog 1 (Drosophila) SNAIL, Drosophila, homolog of, 1 SNAIL1 Zinc finger protein SNAI1 |
Images
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Fig1:
Western blot analysis of SNAIL on different lysates with Rabbit anti-SNAIL antibody (ER1706-22) at 1/5,000 dilution. Lane 1: A549 cell lysate Lane 2: 293T cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 29 kDa Observed band size: 29 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-22) at 1/5,000 dilution was used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of NIH/3T3 cells labeling SNAIL with Rabbit anti-SNAIL antibody (ER1706-22) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAIL antibody (ER1706-22) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.