PRTN3 Rabbit Polyclonal Antibody
cat.: ER1706-25
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PRTN3 aa 50-248 / 256. |
| Positive control: | U937, mouse marrow lysate, 293T, A549, SH-SY5Y, human spleen tissue. |
| Subcellular location: | Cytoplasmic granule, Secreted, Cell membrane, Membrane raft. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50-1:200 1:500 |
| Uniprot #: | SwissProt: P24158 Human | Q61096 Mouse |
| Alternative names: | ACPA AGP 7 AGP7 AGP7 serine proteinase Azurophil Granule Protein 7 C ANCA C ANCA antigen C-ANCA antigen CANCA EC 3.4.21.76 Leukocyte proteinase 3 MBN MBT MBT WEGENER AUTOANTIGEN Myeloblastin Neutrophil proteinase 4 NP 4 NP-4 NP4 P29 PR 3 PR-3 PR3 Proteinase 3 Proteinase3 PRTN 3 Prtn3 PRTN3_HUMAN Serine proteinase neutrophil Wegener granulomatosis autoantigen Serine proteinase, neutrophil Wegener autoantigen Wegener granulomatosis autoantigen |
Images
|
Fig1: Western blot analysis of PRTN3 on U937 using anti-PRTN3 antibody at 1/500 dilution. |
|
Fig2: Western blot analysis of PRTN3 on mouse marrow lysate using anti-PRTN3 antibody at 1/100 dilution. |
|
Fig3: ICC staining PRTN3 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig4: ICC staining PRTN3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig5: ICC staining PRTN3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig6:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PRTN3 antibody (ER1706-25) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-25) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.