PRTN3 Rabbit Polyclonal Antibody
cat.: ER1706-25
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PRTN3 aa 50-248 / 256. |
| Positive control: | HL-60 cell lysate, human spleen tissue. |
| Subcellular location: | Cytoplasmic granule, Secreted, Cell membrane, Membrane raft. |
| Recommended Dilutions:
WB IHC-P |
1:1000 1:500 |
| Uniprot #: | SwissProt: P24158 Human | Q61096 Mouse |
| Alternative names: | ACPA AGP 7 AGP7 AGP7 serine proteinase Azurophil Granule Protein 7 C ANCA C ANCA antigen C-ANCA antigen CANCA EC 3.4.21.76 Leukocyte proteinase 3 MBN MBT MBT WEGENER AUTOANTIGEN Myeloblastin Neutrophil proteinase 4 NP 4 NP-4 NP4 P29 PR 3 PR-3 PR3 Proteinase 3 Proteinase3 PRTN 3 Prtn3 PRTN3_HUMAN Serine proteinase neutrophil Wegener granulomatosis autoantigen Serine proteinase, neutrophil Wegener autoantigen Wegener granulomatosis autoantigen |
Images
|
Fig1:
Western blot analysis of PRTN3 on HL-60 (Human acute promyelocytic leukemia cell) cell lysate with Rabbit anti-PRTN3 antibody (ER1706-25) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 180 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1706-25, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 27 kDa Observed band size: 27 kDa |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-PRTN3 antibody (ER1706-25) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-25) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.