PINK1 Rabbit Polyclonal Antibody
cat.: ER1706-27
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PINK1 aa 94-581 / 581. |
| Positive control: | HeLa treated with 10μM CCCP for 24 hours cell lysate. |
| Subcellular location: | Mitochondrion outer membrane, Mitochondrion inner membrane, Cytoplasm, cytosol. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: Q9BXM7 Human |
| Alternative names: | BRPK FLJ27236 mitochondrial PARK 6 PARK6 Phosphatase and Tensin Homolog PINK 1 PINK1 PINK1_HUMAN Protein kinase BRPK PTEN induced putative kinase 1 PTEN induced putative kinase protein 1 PTEN-induced putative kinase protein 1 Serine/threonine kinase PINK1 mitochondrial Serine/threonine protein kinase PINK1 mitochondrial Serine/threonine-protein kinase PINK1 |
Images
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Fig1:
Western blot analysis of PINK1 on different lysates with Rabbit anti-PINK1 antibody (ER1706-27) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 10μM CCCP for 24 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-27) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.