PINK1 Rabbit Polyclonal Antibody
cat.: ER1706-27
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 63 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PINK1 aa 126-320 / 581. |
| Positive control: | Mouse brain tissue lysate, mouse stomach tissue lysate, mouse placenta tissue lysate, C6 cell lysate, mouse testis tissue lysate, rat stomach tissue lysate, rat testis tissue lysate, Hela, PC-3M, SK-Br-3, human breast tissue, human placenta tissue, human stomach cancer tissue. |
| Subcellular location: | Mitochondrion outer membrane. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:50-1:200 1:100-1:200 |
| Uniprot #: | SwissProt: Q9BXM7 Human | Q99MQ3 Mouse Entrez Gene: 298575 Rat |
| Alternative names: | BRPK FLJ27236 mitochondrial PARK 6 PARK6 Phosphatase and Tensin Homolog PINK 1 PINK1 PINK1_HUMAN Protein kinase BRPK PTEN induced putative kinase 1 PTEN induced putative kinase protein 1 PTEN-induced putative kinase protein 1 Serine/threonine kinase PINK1 mitochondrial Serine/threonine protein kinase PINK1 mitochondrial Serine/threonine-protein kinase PINK1 |
Images
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Fig1:
Western blot analysis of PINK1 on different lysates with Rabbit anti-PINK1 antibody (ER1706-27) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse stomach tissue lysate Lane 3: Mouse placenta tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-27) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of PINK1 on different lysates with Rabbit anti-PINK1 antibody (ER1706-27) at 1/1,000 dilution. Lane 1: C6 cell lysate Lane 2: Mouse testis tissue lysate Lane 3: Rat stomach tissue lysate Lane 4: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 63 kDa Observed band size: 63 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-27) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining PINK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PINK1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining PINK1 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PINK1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PINK1 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-PINK1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.