TGM6 Rabbit Polyclonal Antibody
cat.: ER1706-28
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 79/71 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to N-terminal of Mouse TGM6 |
| Positive control: | 293T, A549, SH-SY5Y, rat brain tissue, human kidney tissue, mouse testis tissue, mouse brain tissue, mouse spleen tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O95932 Human | Q6Y684 Mouse | F1M4T7 Rat |
| Alternative names: | Protein-glutamine gamma-glutamyltransferase 6 TG6 TGase Y TGase-3-like TGase-6 TGM3L_HUMAN TGM6 TGY Transglutaminase Y Transglutaminase-3-like Transglutaminase-6 |
Images
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Fig1: ICC staining TGM6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining TGM6 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining TGM6 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGM6 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TGM6 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TGM6 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TGM6 antibody. Counter stained with hematoxylin. |
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Fig8:
Western blot analysis of TGM6 on different lysates with Rabbit anti-TGM6 antibody (ER1706-28) at 1/1,000 dilution. Lane 1: MOLT-4 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: Mouse kidney tissue lysate Lane 4: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 180 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1706-28, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 79 kDa Observed band size: 71 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.