TGM6 Rabbit Polyclonal Antibody
cat.: ER1706-28
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 79/71 kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to N-terminal of Mouse TGM6
Positive control: 293T, A549, SH-SY5Y, rat brain tissue, human kidney tissue, mouse testis tissue, mouse brain tissue, mouse spleen tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O95932 Human | Q6Y684 Mouse | F1M4T7 Rat
Alternative names: Protein-glutamine gamma-glutamyltransferase 6 TG6 TGase Y TGase-3-like TGase-6 TGM3L_HUMAN TGM6 TGY Transglutaminase Y Transglutaminase-3-like Transglutaminase-6
Images
ER1706-28_1.jpg Fig1: Western blot analysis of TGM6 on different tissue lysates using anti-TGM6 antibody at 1/100 dilution.
Positive control:
Lane 1 : Mouse brain
Lane 2 : Mouse brain
Lane 3 : Mouse spleen
Lane 4 : Mouse spleen
Lane 5 : Mouse testis
ER1706-28_2.jpg Fig2: ICC staining TGM6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-28_3.jpg Fig3: ICC staining TGM6 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-28_4.jpg Fig4: ICC staining TGM6 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-28_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGM6 antibody. Counter stained with hematoxylin.
ER1706-28_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TGM6 antibody. Counter stained with hematoxylin.
ER1706-28_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TGM6 antibody. Counter stained with hematoxylin.
ER1706-28_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TGM6 antibody. Counter stained with hematoxylin.
ER1706-28_9.jpg Fig9: Flow cytometric analysis of 293T cells with TGM6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.