Anti-DLL4 antibody
cat.: ER1706-29
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 74 kDa (Predicted band size)
Isotype: IgG
Immunogen: Recombinant protein within human DLL4 aa 135-370.
Positive control: Mouse placenta tissue lysate, HUVEC, 293T, PMVEC, human liver tissue, human kidney tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9NR61 Human | Q9JI71 Mouse | D3ZHH1 Rat
Alternative names: AOS6 antibody Delta 4 antibody delta 4 precursor antibody Delta ligand 4 antibody delta ligand 4 precursor antibody Delta like 4 antibody Delta like 4 homolog antibody Delta like 4 protein antibody Delta like canonical Notch ligand 4 antibody Delta like protein 4 antibody Delta-like 4 (Drosophila) antibody Delta-like protein 4 antibody Delta4 antibody DLL 4 antibody Dll4 antibody DLL4_HUMAN antibody Drosophila Delta homolog 4 antibody hdelta2 antibody Homeobox protein DLL-4 antibody MGC126344 antibody Notch ligand delta 2 antibody Notch ligand DLL4 antibody Notch ligand DLL4 precursor antibody XDLL-4 antibody
Images
ER1706-29_1.jpg Fig1: Western blot analysis of DLL4 on mouse placenta tissue (1) and HUVEC (2) lysate using anti-DLL4 antibody at 1/500 dilution.
ER1706-29_2.jpg Fig2: ICC staining DLL4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-29_3.jpg Fig3: ICC staining DLL4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-29_4.jpg Fig4: ICC staining DLL4 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-29_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-DLL4 antibody. Counter stained with hematoxylin.
ER1706-29_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DLL4 antibody. Counter stained with hematoxylin.
ER1706-29_7.jpg Fig7: Flow cytometric analysis of HUVEC cells with DLL4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.