NaV1.8 Rabbit Polyclonal Antibody
cat.: ER1706-30
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 220 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rat NaV18 aa 1,907-1,956 / 1,956. |
| Positive control: | Mouse brain tissue, Rat Dorsal root ganglia. |
| Subcellular location: | Plasma membrane. |
| Recommended Dilutions:
IHC-P |
1:500 |
| Uniprot #: | SwissProt: Q6QIY3 Mouse | Q62968 Rat |
| Alternative names: | hPN3 mPN3 Peripheral nerve sodium channel 3 Pn3 (gene name) PN3 SCN10A SCNAA_HUMAN Sensory neuron sodium channel Sns (gene name) SNS Sodium channel protein type 10 subunit alpha Sodium channel protein type X alpha subunit Sodium channel protein type X subunit alpha Voltage-gated sodium channel alpha subunit Nav1.8 Voltage-gated sodium channel subunit alpha Nav1.8 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NaV1.8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2:
Application: Immunohistochemistry (IHC-P) Species: Rat Tissue: Dorsal root ganglia Sample: Paraffin-embedded section Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95℃. Wash buffer: 1× TBST Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. Primary antibody: ER1706-30, 1/500, 1 hour at room temperature. Secondary antibody: HA1119, 20 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.