Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Mouse, Rat |
Applications: | WB, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 220 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within rat NaV18 aa 1,907-1,956 / 1,956. |
Positive control: | Rat spinal cord lysates, mouse brain tissue, mouse brain tissue lysates. |
Subcellular location: | Plasma membrane. |
Recommended Dilutions:
WB IHC-P |
1:100-1:1,000 1:50-1:200 |
Uniprot #: | SwissProt: Q6QIY3 Mouse | Q62968 Rat |
Alternative names: | hPN3 mPN3 Peripheral nerve sodium channel 3 Pn3 (gene name) PN3 SCN10A SCNAA_HUMAN Sensory neuron sodium channel Sns (gene name) SNS Sodium channel protein type 10 subunit alpha Sodium channel protein type X alpha subunit Sodium channel protein type X subunit alpha Voltage-gated sodium channel alpha subunit Nav1.8 Voltage-gated sodium channel subunit alpha Nav1.8 |
Fig1: Western blot analysis of NaV1.8 on rat spinal cord lysate using anti-NaV1.8 antibody at 1/100 dilution. | |
Fig2:
Western blot analysis of NaV1.8 on mouse brain tissue lysates with Rabbit anti-NaV1.8 antibody (ER1706-30) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 220 kDa Observed band size: 220 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-30) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NaV1.8 antibody. Counter stained with hematoxylin. |