NaV1.8 Rabbit Polyclonal Antibody
cat.: ER1706-30
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 220 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within rat NaV18 aa 1,907-1,956 / 1,956. |
| Positive control: | Mouse brain tissue lysates, rat spinal cord lysates, mouse brain tissue. |
| Subcellular location: | Plasma membrane. |
| Recommended Dilutions:
WB IHC-P |
1:100-1:1,000 1:500 |
| Uniprot #: | SwissProt: Q6QIY3 Mouse | Q62968 Rat |
| Alternative names: | hPN3 mPN3 Peripheral nerve sodium channel 3 Pn3 (gene name) PN3 SCN10A SCNAA_HUMAN Sensory neuron sodium channel Sns (gene name) SNS Sodium channel protein type 10 subunit alpha Sodium channel protein type X alpha subunit Sodium channel protein type X subunit alpha Voltage-gated sodium channel alpha subunit Nav1.8 Voltage-gated sodium channel subunit alpha Nav1.8 |
Images
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Fig1:
Western blot analysis of NaV1.8 on mouse brain tissue lysates with Rabbit anti-NaV1.8 antibody (ER1706-30) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 220 kDa Observed band size: 220 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-30) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: Western blot analysis of NaV1.8 on rat spinal cord lysate using anti-NaV1.8 antibody at 1/100 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NaV1.8 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-30, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.