Prostate-Specific Antigen (PSA) Rabbit Polyclonal Antibody
cat.: ER1706-31
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 29 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human Prostate-Specific Antigen. |
| Positive control: | Human prostate tissue, human prostate cancer tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P |
1:200 |
| Uniprot #: | SwissProt: P07288 Human |
| Alternative names: | antigen, prostate-specific APS Gamma seminoprotein Gamma-seminoprotein hK3 Kallikrein 3 Kallikrein related peptidase 3 Kallikrein-3 KLK 3 KLK2A1 Klk3 KLK3_HUMAN P-30 antigen P30 antigen Prostate-specific antigen Psa Semenogelase Seminin |
Images
|
Fig1:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-Prostate-Specific Antigen (PSA) antibody (ER1706-31) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-31) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue with Rabbit anti-Prostate-Specific Antigen (PSA) antibody (ER1706-31) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-31) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.