TNFAIP3 Rabbit Polyclonal Antibody
cat.: ER1706-32
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 90 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TNFAIP3 aa 225-410 / 790. |
| Positive control: | Jurkat, Daudi, A431, LOVO, rat lung tissue, human liver cancer tissue, human kidney tissue, mouse testis tissue, HepG2. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P21580 Human | Q60769 Mouse | M0R7V5 Rat |
| Alternative names: | A20 AISBL MGC104522 MGC138687 MGC138688 OTU domain containing protein 7C OTU domain-containing protein 7C OTUD7C Putative DNA binding protein A20 Putative DNA-binding protein A20 TNAP3_HUMAN TNF alpha-induced protein 3 TNFA1P2 TNFAIP 3 TNFAIP3 (A20) TNFAIP3 Tumor necrosis factor alpha induced protein 3 Tumor necrosis factor alpha-induced protein 3 Tumor necrosis factor induced protein 3 Tumor necrosis factor inducible protein A20 tumor necrosis factor, alpha-induced protein 3 Zinc finger protein A20 |
Images
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Fig1: Western blot analysis of TNFAIP3 on Jurkat (1) and Daudi (2) cell lysate using anti-TNFAIP3 antibody at 1/100 dilution. |
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Fig2: ICC staining TNFAIP3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining TNFAIP3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-TNFAIP3 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TNFAIP3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TNFAIP3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-TNFAIP3 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of HepG2 cells with TNFAIP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.