ATF6 Rabbit Polyclonal Antibody
cat.: ER1706-34
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze/thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 75 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ATF6 aa 168-392 / 670. |
| Positive control: | Hela, A431, HUVEC, K562, rat brain tissue, human kidney tissue. |
| Subcellular location: | Endoplasmic reticulum membrane. Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. THBS4 promotes its nuclear shuttling. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P18850 Human |
| Alternative names: | Activating transcription factor 6 alpha Activating transcription factor 6 ATF 6 ATF6 alpha ATF6 ATF6-alpha ATF6A ATF6A_HUMAN cAMP dependent transcription factor ATF 6 alpha cAMP-dependent transcription factor ATF-6 alpha Cyclic AMP dependent transcription factor ATF 6 alpha DKFZp686P2194 ESTM49 FLJ21663 Processed cyclic AMP dependent transcription factor ATF 6 alpha Processed cyclic AMP-dependent transcription factor ATF-6 alpha |
Images
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Fig1: Western blot analysis of ATF6 on Hela cell lysate using anti-ATF6 antibody at 1/500 dilution. |
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Fig2: ICC staining ATF6 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining ATF6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATF6 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ATF6 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of K562 cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.