CD71 Rabbit Polyclonal Antibody
cat.: ER1706-35
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 84 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human CD71 aa 463-750 / 760.
Positive control: A549, MCF-7, PC-3M, human kidney tissue, human placenta tissue, mouse brain tissue, mouse kidney tissue, HL-60.
Subcellular location: Secreted and Cell membrane. Melanosome.
Recommended Dilutions:
  ICC
  IHC-P
  FC

1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P02786 Human | Q62351 Mouse
Alternative names: CD 71 CD71 CD71 antigen IMD46 OTTHUMP00000208523 OTTHUMP00000208524 OTTHUMP00000208525 p90 sTfR T9 TFR 1 TfR TfR1 TFR1_HUMAN TFRC TR Transferrin receptor (p90 CD71) Transferrin receptor protein 1, serum form Trfr
Images
ER1706-35_1.jpg Fig1: ICC staining CD71 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-35_2.jpg Fig2: ICC staining CD71 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-35_3.jpg Fig3: ICC staining CD71 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-35_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD71 antibody. Counter stained with hematoxylin.
ER1706-35_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD71 antibody. Counter stained with hematoxylin.
ER1706-35_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD71 antibody. Counter stained with hematoxylin.
ER1706-35_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD71 antibody. Counter stained with hematoxylin.
ER1706-35_8.jpg Fig8: Flow cytometric analysis of HL-60 cells with CD71 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.