CD71 Rabbit Polyclonal Antibody
cat.: ER1706-35
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 84 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD71 aa 463-750 / 760. |
| Positive control: | A549, MCF-7, PC-3M, human kidney tissue, human placenta tissue, mouse brain tissue, mouse kidney tissue, HL-60. |
| Subcellular location: | Secreted and Cell membrane. Melanosome. |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P02786 Human | Q62351 Mouse |
| Alternative names: | CD 71 CD71 CD71 antigen IMD46 OTTHUMP00000208523 OTTHUMP00000208524 OTTHUMP00000208525 p90 sTfR T9 TFR 1 TfR TfR1 TFR1_HUMAN TFRC TR Transferrin receptor (p90 CD71) Transferrin receptor protein 1, serum form Trfr |
Images
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Fig1: ICC staining CD71 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining CD71 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CD71 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD71 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD71 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CD71 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD71 antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of HL-60 cells with CD71 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.