ER1706-36

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Cell, FC
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

73 kDa

Isotype:

IgG

Immunogen:

Synthetic peptide within mouse Kv14 aa 201-250 / 654.

Positive control:

Rat brain tissue, mouse brain tissue, N2A, SH-SY5Y.

Subcellular location:

Cell membrane.

Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC

1:500-1:2,000
1:50-1:100
1:50-1:200
1:50-1:100

Uniprot #:

SwissProt: Q61423 Mouse | P15385 Rat

Alternative names:

Voltage gated K+ channel HuKII Fetal skeletal muscle potassium channel HBK4 HK1 HPCN2 HUKII Kcna4 KCNA4_HUMAN KCNA4L KCNA8 kv1.4 PCN2 Potassium channel 2 Potassium voltage gated channel shaker related subfamily member 4 Potassium voltage gated channel subfamily A member 4 Potassium voltage-gated channel subfamily A member 4 Rapidly inactivating potassium channel Shaker related potassium channel Kv1.4 Type A potassium channel Voltage gated potassium channel HBK4 Voltage gated potassium channel HK1 Voltage gated potassium channel subunit Kv1.4 Voltage-gated K(+) channel HuKII Voltage-gated potassium channel HBK4 Voltage-gated potassium channel HK1 Voltage-gated potassium channel subunit Kv1.4

	Fig1: Western blot analysis of Kv1.4 on rat brain (1) and mouse brain (2) tissue lysate using anti-Kv1.4 antibody at 1/1,000 dilution.
	Fig2: ICC staining Kv1.4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining Kv1.4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Kv1.4 antibody. Counter stained with hematoxylin.

	Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Kv1.4 antibody. Counter stained with hematoxylin.
	Fig6: Flow cytometric analysis of SH-SY5Y cells with Kv1.4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.