KCNA4 Rabbit Polyclonal Antibody
cat.: ER1706-36
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 73 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within mouse Kv14 aa 201-250 / 654. |
| Positive control: | HEK-293 cell lysate,PANC-1 cell lysate, SHG-44 cell lysate, N2A, SH-SY5Y, rat brain, mouse brain. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:500-1:2,000 1:50-1:100 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q61423 Mouse | P15385 Rat |
| Alternative names: | Voltage gated K+ channel HuKII Fetal skeletal muscle potassium channel HBK4 HK1 HPCN2 HUKII Kcna4 KCNA4_HUMAN KCNA4L KCNA8 kv1.4 PCN2 Potassium channel 2 Potassium voltage gated channel shaker related subfamily member 4 Potassium voltage gated channel subfamily A member 4 Potassium voltage-gated channel subfamily A member 4 Rapidly inactivating potassium channel Shaker related potassium channel Kv1.4 Type A potassium channel Voltage gated potassium channel HBK4 Voltage gated potassium channel HK1 Voltage gated potassium channel subunit Kv1.4 Voltage-gated K(+) channel HuKII Voltage-gated potassium channel HBK4 Voltage-gated potassium channel HK1 Voltage-gated potassium channel subunit Kv1.4 |
Images
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Fig1:
Western blot analysis of KCNA4 on different lysates with Rabbit anti-KCNA4 antibody (ER1706-36) at 1/1,000 dilution. Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate Lane 2: PANC-1 (Human epidermoid carcinoma pancreatic ductal cell) cell lysate Lane 3: SHG-44 (Human glioma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 59 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1706-36, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 73 kDa Observed band size: 73 kDa |
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Fig2: ICC staining Kv1.4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Kv1.4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Kv1.4 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Kv1.4 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.