PPM1A Rabbit Polyclonal Antibody
cat.: ER1706-37
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PP2C alpha aa 42-330 / 382. |
| Positive control: | HeLa cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, HeLa, human heart tissue, mouse heart tissue, rat heart tissue. |
| Subcellular location: | Nucleus, Cytoplasm, cytosol, Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000 1:100 1:200 |
| Uniprot #: | SwissProt: P35813 Human | P49443 Mouse | P20650 Rat |
| Alternative names: | Protein phosphatase 2C isoform alpha EC 3.1.3.16 FLJ42306 IA MGC9201 Mpp alpha PP2C alpha PP2C-alpha PP2CA PP2Calpha PPM 1A PPM1A PPM1A_HUMAN PPPM1A Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform Protein phosphatase 1A Protein phosphatase 1A magnesium dependent alpha Protein phosphatase 2C alpha Protein phosphatase 2C alpha isoform Protein phosphatase 2C isoform alpha Protein phosphatase IA Protein phosphatase, Mg2+/Mn2+ dependent, 1A |
Images
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Fig1:
Western blot analysis of PPM1A on different lysates with Rabbit anti-PPM1A antibody (ER1706-37) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: Mouse brain tissue lysate (40 µg/Lane) Lane 3: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 23 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-37) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling PPM1A with Rabbit anti-PPM1A antibody (ER1706-37) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PPM1A antibody (ER1706-37) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human heart tissue with Rabbit anti-PPM1A antibody (ER1706-37) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-37) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-PPM1A antibody (ER1706-37) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-37) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat heart tissue with Rabbit anti-PPM1A antibody (ER1706-37) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-37) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.