PPM1A Rabbit Polyclonal Antibody
cat.: ER1706-37
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 42/35 kDa, additional band 55 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human PP2C alpha aa 42-330 / 382. |
| Positive control: | Mouse kidney tissue lysate, rat skin tissue lysate, A431, A549, SH-SY5Y, human kidney tissue, human colon cancer tissue, mouse intestine tissue. |
| Subcellular location: | Cytoplasm, Membrane, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P35813 Human | P49443 Mouse | P20650 Rat |
| Alternative names: | Protein phosphatase 2C isoform alpha EC 3.1.3.16 FLJ42306 IA MGC9201 Mpp alpha PP2C alpha PP2C-alpha PP2CA PP2Calpha PPM 1A PPM1A PPM1A_HUMAN PPPM1A Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform Protein phosphatase 1A Protein phosphatase 1A magnesium dependent alpha Protein phosphatase 2C alpha Protein phosphatase 2C alpha isoform Protein phosphatase 2C isoform alpha Protein phosphatase IA Protein phosphatase, Mg2+/Mn2+ dependent, 1A |
Images
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Fig1: Western blot analysis of PP2C alpha on mouse kidney tissue (1) and rat skin tissue (2) lysate using anti-PP2C alpha antibody at 1/100 dilution. |
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Fig2: ICC staining PP2C alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PP2C alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PP2C alpha in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PP2C alpha antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PP2C alpha antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PP2C alpha antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of A431 cells with PP2C alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.