Cdc25C Rabbit Polyclonal Antibody
cat.: ER1706-38
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 53 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within C-terminal human Cdc25C. |
| Positive control: | Human skin tissue lysate, A431, HUVEC, LOVO, rat brain tissue, human placenta tissue, mouse uterus tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P30307 Human | P48967 Mouse Unigene: 162298 Rat |
| Alternative names: | CDC 25 Cdc 25C CDC25 CDC25C Cell division cycle 25 homolog C Cell division cycle 25C Cell division cycle 25C protein Dual specificity phosphatase Cdc25C M phase inducer phosphatase 3 M-phase inducer phosphatase 3 Mitosis inducer CDC25 MPIP3 MPIP3_HUMAN Phosphotyrosine phosphatase PPP1R60 protein phosphatase 1, regulatory subunit 60 |
Images
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Fig1: Western blot analysis of Cdc25C on human skin tissue lysate using anti-Cdc25C antibody at 1/500 dilution. |
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Fig2: ICC staining Cdc25C in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Cdc25C in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Cdc25C in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Cdc25C antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-Cdc25C antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cdc25C antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of LOVO cells with Cdc25C antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.