GOLPH3 Rabbit Polyclonal Antibody
cat.: ER1706-39
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 34 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal human GOLPH3. |
| Positive control: | Hela, HepG2, SK-Br-3, rat epididymis tissue, human stomach cancer tissue, mouse stomach tissue. |
| Subcellular location: | Cell membrane. Endosome. Cytoplasm. |
| Recommended Dilutions:
IF-Cell IHC-P FC WB |
1:50-1:200 1:50-1:200 1:50-1:100 1:500 |
| Uniprot #: | SwissProt: Q9H4A6 Human | Q9CRA5 Mouse | Q9ERE4 Rat |
| Alternative names: | Coat protein Coat protein GPP34 FLJ90675 Golgi associated protein Golgi peripheral membrane protein 1, 34 kDa Golgi phosphoprotein 3 (coat protein) Golgi phosphoprotein 3 Golgi protein GOLP3_HUMAN Golph3 GOPP1 GPP34 MIDAS Mitochondrial DNA absence factor |
Images
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Fig1: ICC staining GOLPH3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining GOLPH3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining GOLPH3 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GOLPH3 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-GOLPH3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-GOLPH3 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Hela cells with GOLPH3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.