GOLPH3 Rabbit Polyclonal Antibody
cat.: ER1706-39
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 34 kDa
Isotype: IgG
Immunogen: Recombinant protein within C-terminal human GOLPH3.
Positive control: Hela, HepG2, SK-Br-3, rat epididymis tissue, human stomach cancer tissue, mouse stomach tissue.
Subcellular location: Cell membrane. Endosome. Cytoplasm.
Recommended Dilutions:
  IF-Cell
  IHC-P
  FC
  WB

1:50-1:200
1:50-1:200
1:50-1:100
1:500
Uniprot #: SwissProt: Q9H4A6 Human | Q9CRA5 Mouse | Q9ERE4 Rat
Alternative names: Coat protein Coat protein GPP34 FLJ90675 Golgi associated protein Golgi peripheral membrane protein 1, 34 kDa Golgi phosphoprotein 3 (coat protein) Golgi phosphoprotein 3 Golgi protein GOLP3_HUMAN Golph3 GOPP1 GPP34 MIDAS Mitochondrial DNA absence factor
Images
ER1706-39_1.jpg Fig1: ICC staining GOLPH3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-39_2.jpg Fig2: ICC staining GOLPH3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-39_3.jpg Fig3: ICC staining GOLPH3 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-39_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GOLPH3 antibody. Counter stained with hematoxylin.
ER1706-39_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-GOLPH3 antibody. Counter stained with hematoxylin.
ER1706-39_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-GOLPH3 antibody. Counter stained with hematoxylin.
ER1706-39_7.jpg Fig7: Flow cytometric analysis of Hela cells with GOLPH3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.