Anti-MMP9 antibody
cat.: ER1706-40
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 78 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human MMP9 aa 105-179.
Positive control: U937 cell lysates, Hela, MCF-7, PANC-1, human spleen tissue, mouse spleen tissue, human tonsil tissue, HL-60.
Subcellular location: Extracellular matrix. Secreted.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P14780 Human | P41245 Mouse
Alternative names: 82 kDa matrix metalloproteinase-9 92 kDa gelatinase 92 kDa type IV collagenase CLG 4B CLG4B Collagenase Type 4 beta Collagenase type IV 92 KD EC 3.4.24.35 Gelatinase 92 KD Gelatinase B Gelatinase beta GelatinaseB GELB Macrophage gelatinase MANDP2 Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) Matrix Metalloproteinase 9 MMP 9 MMP-9 MMP9 MMP9_HUMAN Type V collagenase
Images
ER1706-40_1.jpg Fig1: Western blot analysis of MMP9 on U937 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1706-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1706-40_2.jpg Fig2: ICC staining of MMP9 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1706-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1706-40_3.jpg Fig3: ICC staining of MMP9 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1706-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1706-40_4.jpg Fig4: ICC staining of MMP9 in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1706-40, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1706-40_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-MMP9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1706-40_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-MMP9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1706-40_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MMP9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-40, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1706-40_8.jpg Fig8: Flow cytometric analysis of MMP9 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1706-40, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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