TLR4 Rabbit Polyclonal Antibody
cat.: ER1706-43
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 96/91/73 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human TLR4 aa 766-820.
Positive control: Hela, HUVEC, PMVEC, rat kidney tissue, human skin tissue, human spleen tissue, mouse colon tissue, Jurkat.
Subcellular location: Cell membrane.
Recommended Dilutions:
  IF-Cell
  IHC-P
  FC

1:50-1:200
1:50-1:500
1:50-1:100
Uniprot #: SwissProt: O00206 Human | Q9QUK6 Mouse | Q9QX05 Rat
Alternative names: ARMD10 CD284 CD284 antigen Homolog of Drosophila toll hToll TLR 4 TLR4 TLR4_HUMAN TOLL Toll like receptor 4 Toll-like receptor 4
Images
ER1706-43_1.jpg Fig1: ICC staining TLR4 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-43_2.jpg Fig2: ICC staining TLR4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-43_3.jpg Fig3: ICC staining TLR4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-43_4.png Fig4: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-TLR4 antibody (ER1706-43) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-43) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1706-43_5.png Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-TLR4 antibody (ER1706-43) at 1/500 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-43) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1706-43_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-TLR4 antibody. Counter stained with hematoxylin.
ER1706-43_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TLR4 antibody. Counter stained with hematoxylin.
ER1706-43_8.jpg Fig8: Flow cytometric analysis of Jurkat cells with TLR4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.