NLRC3 Rabbit Polyclonal Antibody
cat.: ER1706-45
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 120 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NLRC3 aa 1-50 / 1,065. |
| Positive control: | Human thymus tissue lysates, Raji, human tonsil tissue, human colon cancer tissue, human spleen tissue, mouse colon tissue. |
| Subcellular location: | Cytoplasm. Cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:200 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: Q7RTR2 Human | Q5DU56 Mouse |
| Alternative names: | CARD15-like CARD15-like protein Caterpiller 16.2 Caterpiller protein 16.2 CLR16.2 FLJ00348 NLR family CARD domain containing 3 Nlrc3 NLRC3_HUMAN NOD-like receptor C3 NOD3 NOD3 protein Nucleotide-binding oligomerization domain protein 3 Nucleotide-binding oligomerization domain protein 3 caterpiller 16.2 nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3 Protein NLRC3 |
Images
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Fig1: Western blot analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution. |
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Fig2:
Immunocytochemistry analysis of Raji cells labeling NLRC3 with Rabbit anti-NLRC3 antibody (ER1706-45) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NLRC3 antibody (ER1706-45) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NLRC3 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NLRC3 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NLRC3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NLRC3 antibody. Counter stained with hematoxylin. |
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Fig7:
Flow cytometric analysis of Raji cells labeling NLRC3. Cells were fixed and permeabilized. Then stained with the primary antibody (ER1706-45, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.