NLRC3 Rabbit Polyclonal Antibody
cat.: ER1706-45
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 120 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NLRC3 aa 1-50 / 1,065. |
| Positive control: | Human thymus tissue lysates, HUVEC, MCF-7, SH-SY5Y, human tonsil tissue, human colon cancer tissue, human spleen tissue, mouse colon tissue. |
| Subcellular location: | Cytoplasm. Cytoskeleton. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q7RTR2 Human | Q5DU56 Mouse |
| Alternative names: | CARD15-like CARD15-like protein Caterpiller 16.2 Caterpiller protein 16.2 CLR16.2 FLJ00348 NLR family CARD domain containing 3 Nlrc3 NLRC3_HUMAN NOD-like receptor C3 NOD3 NOD3 protein Nucleotide-binding oligomerization domain protein 3 Nucleotide-binding oligomerization domain protein 3 caterpiller 16.2 nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 3 Protein NLRC3 |
Images
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Fig1: Western blot analysis of NLRC3 on human thymus tissue lysates using anti-NLRC3 antibody at 1/500 dilution. |
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Fig2: ICC staining NLRC3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining NLRC3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining NLRC3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NLRC3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NLRC3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NLRC3 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NLRC3 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of MCF-7 cells with NLRC3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.