Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 359 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within human Ki67 aa 1009-1094. |
Positive control: | HepG2, A431, A549, LOVO, human tonsil tissue, human lung cancer tissue, human colon cancer tissue, human stomach cancer tissue. |
Subcellular location: | Nucleus. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1,000 1:200-1:1,000 1:100-1:500 1:50-1:200 |
Uniprot #: | SwissProt: P46013 Human |
Alternative names: | Antigen identified by monoclonal Ki 67 Antigen identified by monoclonal Ki-67 Antigen KI-67 Antigen KI67 Antigen Ki67 KI67_HUMAN KIA Marker of proliferation Ki-67 MIB 1 MIB MKI67 PPP1R105 Proliferation marker protein Ki-67 Proliferation related Ki 67 antigen Protein phosphatase 1 regulatory subunit 105 RP11-380J17.2 |
Fig1: ICC staining Ki67 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig2: ICC staining Ki67 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining Ki67 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. |
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. | |
Fig7: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Ki67 antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. | |
Fig8: Flow cytometric analysis of Hela cells with Ki67 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Fig9: Western blot analysis of Ki67 on HepG2 cell lysate using anti-Ki67 antibody at 1/1,000 dilution. |