Anti-BCL2 antibody
cat.: ER1706-47
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 26 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human BCL2 aa 20-74.
Positive control: Hela, THP-1, HepG2, MCF-7, SH-SY5Y.
Subcellular location: Endoplasmic reticulum. Mitochondrion. Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:100-1:200
1:50-1:200
1:50-1:100
Uniprot #: P10415(Human)
Alternative names: Apoptosis regulator Bcl 2 antibody Apoptosis regulator Bcl-2 antibody Apoptosis regulator Bcl2 antibody AW986256 antibody B cell CLL/lymphoma 2 antibody B cell leukemia/lymphoma 2 antibody Bcl-2 antibody Bcl2 antibody BCL2_HUMAN antibody C430015F12Rik antibody D630044D05Rik antibody D830018M01Rik antibody Leukemia/lymphoma, B-cell, 2 antibody Oncogene B-cell leukemia 2 antibody PPP1R50 antibody Protein phosphatase 1, regulatory subunit 50 antibody
Images
ER1706-47_1.jpg Fig1: Western blot analysis of BCL2 on Hela cell lysate using anti-BCL2 antibody at 1/1,000 dilution.
ER1706-47_2.jpg Fig2: ICC staining BCL2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-47_3.jpg Fig3: ICC staining BCL2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-47_4.jpg Fig4: ICC staining BCL2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-47_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-BCL2 antibody. Counter stained with hematoxylin.
ER1706-47_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-BCL2 antibody. Counter stained with hematoxylin.
ER1706-47_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-BCL2 antibody. Counter stained with hematoxylin.
ER1706-47_8.jpg Fig8: Flow cytometric analysis of MCF-7 cells with BCL2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.