ROCK2 Rabbit Polyclonal Antibody
cat.: ER1706-48
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 161 kDa,
Isotype: IgG
Immunogen: Recombinant protein within C-terminal human ROCK2.
Positive control: HeLa cell lysate, MCF7 cell lysate, A549 cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, rat brain tissue lysate, C2C12, rat brain tissue, human liver tissue, human colon cancer tissue, mouse brain tissue.
Subcellular location: Cytoplasm, Cell membrane, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:2,000
1:100
1:50-1:200
1:1,000
Uniprot #: SwissProt: O75116 Human | P70336 Mouse | Q62868 Rat
Alternative names: coiled-coil-containing protein kinase 2 KIAA0619 p164 ROCK 2 p164 ROCK-2 Rho associated coiled coil containing protein kinase 2 Rho associated protein kinase 2 Rho associated, coiled coil containing protein kinase II Rho kinase 2 Rho-associated Rho-associated protein kinase 2 ROCK 2 Rock II Rock2 ROCK2_HUMAN Rock2m ROK alpha ROKalpha
Images
ER1706-48_1.jpg Fig1: Western blot analysis of ROCK2 on different lysates with Rabbit anti-ROCK2 antibody (ER1706-48) at 1/2,000 dilution.

Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: HepG2 cell lysate (20 µg/Lane)
Lane 5: NIH/3T3 cell lysate (20 µg/Lane)
Lane 6: C2C12 cell lysate (20 µg/Lane)
Lane 7: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 161 kDa
Observed band size: 161 kDa

Exposure time: 59 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-48) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER1706-48_2.jpg Fig2: Immunocytochemistry analysis of C2C12 cells labeling ROCK2 with Rabbit anti-ROCK2 antibody (ER1706-48) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ROCK2 antibody (ER1706-48) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ER1706-48_3.jpg Fig3: Flow cytometric analysis of C2C12 cells labeling ROCK2.

Cells were fixed and permeabilized. Then stained with the primary antibody (ER1706-48, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ER1706-48_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.
ER1706-48_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.
ER1706-48_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.
ER1706-48_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.