Anti-ROCK2 antibody
cat.: ER1706-48
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 161 kDa
Isotype: IgG
Immunogen: Recombinant protein within C-terminal human ROCK2.
Positive control: Raji, siHa, Hela, HepG2, rat brain tissue, human liver tissue, human colon cancer tissue, mouse brain tissue.
Subcellular location: Plasma membrane. Cytoskeleton. Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500
1:50-1:200
1:50-1:200
Uniprot #: O75116(Human) P70336(Mouse) Q62868(Rat)
Alternative names: coiled-coil-containing protein kinase 2 antibody KIAA0619 antibody p164 ROCK 2 antibody p164 ROCK-2 antibody Rho associated coiled coil containing protein kinase 2 antibody Rho associated protein kinase 2 antibody Rho associated, coiled coil containing protein kinase II antibody Rho kinase 2 antibody Rho-associated antibody Rho-associated protein kinase 2 antibody ROCK 2 antibody Rock II antibody Rock2 antibody ROCK2_HUMAN antibody Rock2m antibody ROK alpha antibody ROKalpha antibody
Images
ER1706-48_1.jpg Fig1: Western blot analysis of ROCK2 on Raji (1) and SiHa (2) cell lysates using anti-ROCK2 antibody at 1/500 dilution.
ER1706-48_2.jpg Fig2: ICC staining ROCK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-48_3.jpg Fig3: ICC staining ROCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-48_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.
ER1706-48_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.
ER1706-48_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.
ER1706-48_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ROCK2 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.