ROCK2 Rabbit Polyclonal Antibody
cat.: ER1706-48
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 161 kDa, |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within C-terminal human ROCK2. |
| Positive control: | Raji, siHa, Hela, HepG2, rat brain tissue, human liver tissue, human colon cancer tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm, Cell membrane, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: O75116 Human | P70336 Mouse | Q62868 Rat |
| Alternative names: | coiled-coil-containing protein kinase 2 KIAA0619 p164 ROCK 2 p164 ROCK-2 Rho associated coiled coil containing protein kinase 2 Rho associated protein kinase 2 Rho associated, coiled coil containing protein kinase II Rho kinase 2 Rho-associated Rho-associated protein kinase 2 ROCK 2 Rock II Rock2 ROCK2_HUMAN Rock2m ROK alpha ROKalpha |
Images
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Fig1: Western blot analysis of ROCK2 on Raji (1) and SiHa (2) cell lysates using anti-ROCK2 antibody at 1/500 dilution. |
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Fig2: ICC staining ROCK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining ROCK2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ROCK2 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-ROCK2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ROCK2 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ROCK2 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.