Superoxide Dismutase 1 Rabbit Polyclonal Antibody
cat.: ER1706-49
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 16 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human SOD1 aa 6-154. |
| Positive control: | MCF7 cell lysate, HepG2 cell lysate, HUVEC cell lysate, SH-SY5Y cell lysate, human liver tissue lysate, HepG2, LOVO, MCF-7. |
| Subcellular location: | Cytoplasm, Mitochondrion, Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: P00441 Human | P08228 Mouse | P07632 Rat |
| Alternative names: | ALS ALS1 Amyotrophic lateral sclerosis 1 adult Cu/Zn SOD Cu/Zn superoxide dismutase Epididymis secretory protein Li 44 HEL S 44 Homodimer hSod1 Indophenoloxidase A IPOA Mn superoxide dismutase SOD SOD soluble SOD1 SOD2 SODC SODC_HUMAN Superoxide dismutase [Cu-Zn] Superoxide dismutase 1 Superoxide dismutase 1 soluble Superoxide dismutase Cu Zn Superoxide dismutase cystolic |
Images
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Fig1:
Western blot analysis of Superoxide Dismutase 1 on different lysates with Rabbit anti-Superoxide Dismutase 1 antibody (ER1706-49) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (20 µg/Lane) Lane 2: HepG2 cell lysate (20 µg/Lane) Lane 3: HUVEC cell lysate (20 µg/Lane) Lane 4: SH-SY5Y cell lysate (20 µg/Lane) Lane 5: Human liver tissue lysate (40 µg/Lane) Predicted band size: 16 kDa Observed band size: 16 kDa Exposure time: 1 second; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-49) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining SOD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining SOD1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Flow cytometric analysis of MCF-7 cells with SOD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.