SOD1 Rabbit Polyclonal Antibody
cat.: ER1706-49
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 16 kDa
Isotype: IgG
Immunogen: Recombinant protein within human SOD1 aa 6-154.
Positive control: A549, HepG2, LOVO, rat testis tissue, human liver tissue, human kidney tissue, mouse brain tissue,MCF-7.
Subcellular location: Cytoplasm, Mitochondrion, Nucleus.
Recommended Dilutions:
  WB
  ICC
  FC

1:500
1:500-1:2,000
1:50-1:100
Uniprot #: SwissProt: P00441 Human | P08228 Mouse | P07632 Rat
Alternative names: ALS ALS1 Amyotrophic lateral sclerosis 1 adult Cu/Zn SOD Cu/Zn superoxide dismutase Epididymis secretory protein Li 44 HEL S 44 Homodimer hSod1 Indophenoloxidase A IPOA Mn superoxide dismutase SOD SOD soluble SOD1 SOD2 SODC SODC_HUMAN Superoxide dismutase [Cu-Zn] Superoxide dismutase 1 Superoxide dismutase 1 soluble Superoxide dismutase Cu Zn Superoxide dismutase cystolic
Images
ER1706-49_1.jpg Fig1: Western blot analysis of SOD1 on A549 cell lysate using anti-SOD1 antibody at 1/500 dilution.
ER1706-49_2.jpg Fig2: ICC staining SOD1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-49_3.jpg Fig3: ICC staining SOD1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-49_4.jpg Fig4: Flow cytometric analysis of MCF-7 cells with SOD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.