PTEN Rabbit Polyclonal Antibody
cat.: ER1706-51
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PTEN aa 70-268. |
| Positive control: | MCF-7, Hela, HUVEC, SH-SY5Y, human thyroid gland tissue, human spleen tissue, mouse brain tissue. |
| Subcellular location: | Nucleus. Cytoplasm. Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P60484 Human | O08586 Mouse |
| Alternative names: | 10q23del BZS DEC GLM2 MGC11227 MHAM MMAC1 MMAC1 phosphatase and tensin homolog deleted on chromosome 10 Mutated in multiple advanced cancers 1 Phosphatase and tensin homolog Phosphatase and tensin like protein Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Pten PTEN_HUMAN PTEN1 TEP1 |
Images
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Fig1: Western blot analysis of PTEN on MCF-7 cell lysates using anti-PTEN antibody at 1/500 dilution. |
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Fig2: ICC staining PTEN in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PTEN in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PTEN in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PTEN antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-PTEN antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PTEN antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of SH-SY5Y cells with PTEN antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.