Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 24 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human HMGB2 aa 22-172. |
Positive control: | Mouse testis tissue, LOVO, SH-SY5Y, rat brain tissue, human tonsil tissue, human kidney tissue. |
Subcellular location: | Secreted. Nucleus. Cytoplasm. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: P26583 Human | P30681 Mouse | P52925 Rat |
Alternative names: | C80539 High mobility group (nonhistone chromosomal) protein 2 High mobility group box 2 High mobility group protein 2 High mobility group protein B2 HMG 2 HMG B2 HMG-2 HMG2 HMGB2 HMGB2_HUMAN |
Fig1: Western blot analysis of HMGB2 on mouse testis tissue lysate using anti-HMGB2 antibody at 1/500 dilution. | |
Fig2: ICC staining HMGB2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining HMGB2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HMGB2 antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HMGB2 antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HMGB2 antibody. Counter stained with hematoxylin. | |
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HMGB2 antibody. Counter stained with hematoxylin. |