LOX-1 Rabbit Polyclonal Antibody
cat.: ER1706-53
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 31 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human LOX-1 aa 17-273 / 273. |
| Positive control: | MCF-7, Hela, LOVO, human lung tissue lysate, human kidney tissue, human placenta tissue, mouse brain tissue. |
| Subcellular location: | Cell membrane. Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P78380 Human | Q9EQ09 Mouse |
| Alternative names: | C-type lectin domain family 8 member A CLEC8A hLOX 1 hLOX-1 Lectin like oxidized LDL receptor 1 Lectin like oxLDL receptor 1 Lectin type oxidized LDL receptor 1 Lectin-like oxidized LDL receptor 1 Lectin-like oxLDL receptor 1 Lectin-type oxidized LDL receptor 1 low density lipoprotein oxidized, receptor 1 LOX-1 LOXIN Olr1 OLR1_HUMAN Ox LDL receptor 1 Ox-LDL receptor 1 Oxidised low density lipoprotein (lectin like) receptor 1 Oxidized low density lipoprotein receptor 1 Oxidized low density lipoprotein receptor 1 soluble form Oxidized low-density lipoprotein receptor 1 SCARE1 Scavenger receptor class E, member 1 SLOX1 soluble form SR-EI |
Images
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Fig1:
Western blot analysis of LOX-1 on MCF-7 cell lysates with Rabbit anti-LOX-1 antibody (ER1706-53) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-53) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining LOX1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining LOX1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LOX1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOX1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LOX1 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of LOVO cells with LOX1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.