Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB, IF-Cell, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 17 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human IL4 aa 25-153. |
Positive control: | Recombinant protein lysate, human spleen tissue, human breast cancer tissue, Jurkat. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:200 1:50-1:600 |
Uniprot #: | SwissProt: P05112 Human |
Alternative names: | B cell growth factor 1 B cell IgG differentiation factor B Cell Stimulatory Factor 1 B-cell stimulatory factor 1 BCGF 1 BCGF1 Binetrakin BSF-1 BSF1 IGG1 induction factor IL 4 IL-4 IL4 IL4_HUMAN Il4e12 Interleukin 4 Interleukin 4 variant 2 Interleukin 4, isoform 1 Interleukin-4 Lymphocyte stimulatory factor 1 MGC79402 Pitrakinra |
Fig1: Western blot analysis of IL4 on recombiant protein lysate using anti-IL4 antibody at 1/1,000 dilution. | |
Fig2: ICC staining pro IL4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-IL4 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL4 antibody. Counter stained with hematoxylin. |