IL-4 Rabbit Polyclonal Antibody
cat.: ER1706-55
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human IL4 aa 25-153. |
| Positive control: | Ramos, human breast cancer tissue, human spleen tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:250 1:50-1:600 |
| Uniprot #: | SwissProt: P05112 Human |
| Alternative names: | B cell growth factor 1 B cell IgG differentiation factor B Cell Stimulatory Factor 1 B-cell stimulatory factor 1 BCGF 1 BCGF1 Binetrakin BSF-1 BSF1 IGG1 induction factor IL 4 IL-4 IL4 IL4_HUMAN Il4e12 Interleukin 4 Interleukin 4 variant 2 Interleukin 4, isoform 1 Interleukin-4 Lymphocyte stimulatory factor 1 MGC79402 Pitrakinra |
Images
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Fig1: Western blot analysis of IL4 on recombiant protein lysate using anti-IL4 antibody at 1/1,000 dilution. |
|
Fig2:
Immunocytochemistry analysis of Ramos cells labeling IL-4 with Rabbit anti-IL-4 antibody (ER1706-55) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL-4 antibody (ER1706-55) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-IL4 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IL4 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.