NaV1.7 Rabbit Polyclonal Antibody
cat.: ER1706-57
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 226 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NaV17 aa 1,939-1,988 / 1,988. |
| Positive control: | SH-SY5Y, A549, Hela, human placenta tissue, rat testis tissue, mouse colon tissue, human liver tissue. |
| Subcellular location: | Plasma membrane. In neurite terminals. |
| Recommended Dilutions:
IF-Cell IHC-P |
1:50-1:200 1:50-1:600 |
| Uniprot #: | SwissProt: Q15858 Human | O08562 Rat |
| Alternative names: | ETHA GEFSP7 hNE Na hNE-Na hNENa NE NA NENA Neuroendocrine sodium channel Peripheral sodium channel 1 PN1 Scn9a SCN9A_HUMAN Sodium channel protein type 9 subunit alpha Sodium channel protein type IX subunit alpha Sodium channel voltage gated type IX alpha Sodium channel voltage gated type IX alpha polypeptide Sodium channel voltage gated type IX alpha subunit Voltage gated sodium channel alpha subunit Nav1.7 Voltage gated sodium channel subunit alpha Nav1 Voltage-gated sodium channel subunit alpha Nav1.7 |
Images
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Fig1: ICC staining NaV1.7 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining NaV1.7 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining NaV1.7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NaV1.7 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NaV1.7 antibody. Counter stained with hematoxylin. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-NaV1.7 antibody (ER1706-57) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-57) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-NaV1.7 antibody (ER1706-57) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-57) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.