MSI2 Rabbit Polyclonal Antibody
cat.: ER1706-59
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, ICC, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1 mg/mL. |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 35/37 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human MSI2 aa 1-185 / 328. |
| Positive control: | SW480, K562, HepG2, PC-12, SH-SY5Y, rat epididymis tissue, human breast tissue, human placenta tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB ICC IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q96DH6 Human | Q920Q6 Mouse | F1LWE6 Rat |
| Alternative names: | FLJ36569 MGC3245 Msi2 MSI2/HOXA9 fusion gene, included MSI2H MSI2H_HUMAN Musashi 2 Musashi homolog 2 Musashi RNA binding protein 2 Musashi, Drosophila, homolog of, 2 Musashi-2 RNA binding protein Musashi homolog 2 RNA-binding protein Musashi homolog 2 WD 40 repeat protein MSI2 |
Images
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Fig1: Western blot analysis of MSI2 on SW480 (1) and K562 (2) cell lysate using anti-MSI2 antibody at 1/500 dilution. |
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Fig2: ICC staining MSI2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining MSI2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining MSI2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-MSI2 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MSI2 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MSI2 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MSI2 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of SH-SY5Y cells with MSI2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.