MSI2 Rabbit Polyclonal Antibody
cat.: ER1706-59
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 35/37 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human MSI2 aa 1-185 / 328.
Positive control: K562, HepG2, PC-12, SH-SY5Y, rat epididymis tissue, human breast tissue, human placenta tissue, mouse brain tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50-1:200
1:50-1:4,000
1:50-1:100
Uniprot #: SwissProt: Q96DH6 Human | Q920Q6 Mouse | F1LWE6 Rat
Alternative names: FLJ36569 MGC3245 Msi2 MSI2/HOXA9 fusion gene, included MSI2H MSI2H_HUMAN Musashi 2 Musashi homolog 2 Musashi RNA binding protein 2 Musashi, Drosophila, homolog of, 2 Musashi-2 RNA binding protein Musashi homolog 2 RNA-binding protein Musashi homolog 2 WD 40 repeat protein MSI2
Images
ER1706-59_1.png Fig1: Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (ER1706-59) at 1/2,000 dilution.

Lane 1: K562 cell lysate
Lane 2: PC-12 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 35/37 kDa
Observed band size: 35/37 kDa

Exposure time: 2 minutes;

12% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-59) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER1706-59_2.jpg Fig2: Western blot analysis of MSI2 on different lysates with Rabbit anti-MSI2 antibody (ER1706-59) at 1/1,000 dilution.

Lane 1: Hela-si NT cell lysate
Lane 2: Hela-si MSI2#1 cell lysate
Lane 3: Hela-si MSI2#2 cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 35 kDa
Observed band size: 35 kDa

Exposure time: 50 seconds;

4-20% SDS-PAGE gel.

ER1706-59 was shown to specifically react with MSI2 in Hela-si NT cells. No bands were observed when Hela-si MSI2 samples were tested. Hela-si NT and Hela-si MSI2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER1706-59, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
ER1706-59_3.png Fig3: ICC staining MSI2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-59_4.jpg Fig4: ICC staining MSI2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-59_5.jpg Fig5: ICC staining MSI2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-59_6.png Fig6: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue with Rabbit anti-MSI2 antibody (ER1706-59) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-59) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1706-59_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
ER1706-59_8.png Fig8: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-MSI2 antibody (ER1706-59) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-59) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1706-59_9.png Fig9: Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MSI2 antibody (ER1706-59) at 1/4,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-59) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1706-59_10.jpg Fig10: Flow cytometric analysis of SH-SY5Y cells with MSI2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.