MSI2 Rabbit Polyclonal Antibody
cat.: ER1706-59
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 35/37 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human MSI2 aa 1-185 / 328.
Positive control: SW480, K562, HepG2, PC-12, SH-SY5Y, rat epididymis tissue, human breast tissue, human placenta tissue, mouse brain tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q96DH6 Human | Q920Q6 Mouse | F1LWE6 Rat
Alternative names: FLJ36569 MGC3245 Msi2 MSI2/HOXA9 fusion gene, included MSI2H MSI2H_HUMAN Musashi 2 Musashi homolog 2 Musashi RNA binding protein 2 Musashi, Drosophila, homolog of, 2 Musashi-2 RNA binding protein Musashi homolog 2 RNA-binding protein Musashi homolog 2 WD 40 repeat protein MSI2
Images
ER1706-59_1.jpg Fig1: Western blot analysis of MSI2 on SW480 (1) and K562 (2) cell lysate using anti-MSI2 antibody at 1/500 dilution.
ER1706-59_2.jpg Fig2: ICC staining MSI2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-59_3.jpg Fig3: ICC staining MSI2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-59_4.jpg Fig4: ICC staining MSI2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-59_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
ER1706-59_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
ER1706-59_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
ER1706-59_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MSI2 antibody. Counter stained with hematoxylin.
ER1706-59_9.jpg Fig9: Flow cytometric analysis of SH-SY5Y cells with MSI2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.