AMACR Rabbit Polyclonal Antibody
cat.: ER1706-60
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human AMACR aa 1-382 / 382. |
| Positive control: | Human kidney tissue, mouse liver tissue lysate, Siha, SW480, HepG2, human liver tissue, human colon cancer tissue, mouse colon tissue, PC-3M. |
| Subcellular location: | Mitochondrion. Peroxisome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:100 1:50-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9UHK6 Human | O09174 Mouse |
| Alternative names: | 2 arylpropionyl CoA epimerase 2 methylacyl CoA racemase 2-methylacyl-CoA racemase Alpha methylacyl CoA racemase Alpha methylacyl Coenzyme A racemase Alpha methylacyl-CoA racemase deficiency, included Alpha-methylacyl-CoA racemase Amacr AMACR deficiency, included AMACR_HUMAN CBAS4 Da1-8 EC 5.1.99.4 Macr1 Methylacyl CoA racemase alpha RACE RM |
Images
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Fig1:
Western blot analysis of AMACR on different lysates using anti-AMACR antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver tissue Lane 2: SiHa Lane 3: SW480 Lane 4: Human kidney tissue |
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Fig2: ICC staining AMACR in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-AMACR antibody (ER1706-60) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-60) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-AMACR antibody. Counter stained with hematoxylin. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-AMACR antibody (ER1706-60) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-60) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-AMACR antibody (ER1706-60) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-60) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Flow cytometric analysis of PC-3M cells with AMACR antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.