| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FOXM1 aa 102-340. |
| Positive control: | A549, HUVEC, LOVO. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
IF-Cell FC WB |
1:500-1:2,000 1:50-1:100 1:500 |
| Uniprot #: | SwissProt: Q08050 Human | O08696 Mouse |
| Alternative names: | FKHL16 Forkhead box M1 Forkhead box protein M1 forkhead like 16 Forkhead-related protein FKHL16 FOX M1 Foxm1 FOXM1_HUMAN FOXM1B Hepatocyte nuclear factor 3 forkhead homolog 11 HFH-11 HFH11 HNF-3/fork-head homolog 11 HNF3 INS1 M phase phosphoprotein 2 M-phase phosphoprotein 2 MPHOSPH2 MPM-2 reactive phosphoprotein 2 MPP2 PIG29 TGT3 Transcription factor Trident Trident WIN Winged-helix factor from INS-1 cells Winged-helix factor from INS1 cells |
|
Fig1: ICC staining FOXM1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining FOXM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining FOXM1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Flow cytometric analysis of LOVO cells with FOXM1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |