DLL4 Rabbit Polyclonal Antibody
cat.: ER1706-63
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 75 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human DLL4 aa 121-159. |
| Positive control: | Human lung tissue lysate, 293T, HUVEC, PMVEC, human kidney tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9NR61 Human | D3ZHH1 Rat |
| Alternative names: | AOS6 Delta 4 delta 4 precursor Delta ligand 4 delta ligand 4 precursor Delta like 4 Delta like 4 homolog Delta like 4 protein Delta like canonical Notch ligand 4 Delta like protein 4 Delta-like 4 (Drosophila) Delta-like protein 4 Delta4 DLL 4 Dll4 DLL4_HUMAN Drosophila Delta homolog 4 hdelta2 Homeobox protein DLL-4 MGC126344 Notch ligand delta 2 Notch ligand DLL4 Notch ligand DLL4 precursor XDLL-4 |
Images
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Fig1: Western blot analysis of DLL4 on human lung tissue lysate using anti-DLL4 antibody at 1/500 dilution. |
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Fig2: ICC staining DLL4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining DLL4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining DLL4 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DLL4 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of 293T cells with DLL4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.