Anti-DLL4 antibody
cat.: ER1706-63
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 74 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human DLL4 aa 121-159.
Positive control: Human lung tissue lysate, 293T, HUVEC, PMVEC, human kidney tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9NR61 Human | D3ZHH1 Rat
Alternative names: AOS6 Delta 4 delta 4 precursor Delta ligand 4 delta ligand 4 precursor Delta like 4 Delta like 4 homolog Delta like 4 protein Delta like canonical Notch ligand 4 Delta like protein 4 Delta-like 4 (Drosophila) Delta-like protein 4 Delta4 DLL 4 Dll4 DLL4_HUMAN Drosophila Delta homolog 4 hdelta2 Homeobox protein DLL-4 MGC126344 Notch ligand delta 2 Notch ligand DLL4 Notch ligand DLL4 precursor XDLL-4
Images
ER1706-63_1.jpg Fig1: Western blot analysis of DLL4 on human lung tissue lysate using anti-DLL4 antibody at 1/500 dilution.
ER1706-63_2.jpg Fig2: ICC staining DLL4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-63_3.jpg Fig3: ICC staining DLL4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-63_4.jpg Fig4: ICC staining DLL4 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-63_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DLL4 antibody. Counter stained with hematoxylin.
ER1706-63_6.jpg Fig6: Flow cytometric analysis of 293T cells with DLL4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.