Anti-PKA C alpha antibody
cat.: ER1706-65
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 40 kDa
Isotype: IgG
Immunogen: Recombinant protein within human PKA C alpha aa 172-338.
Positive control: A431, PC-3M, rat brain tissue, human kidney tissue, mouse testis tissue.
Subcellular location: Mitochondrion. Nucleus.Plasma membrane.
Recommended Dilutions:
  ICC
  IHC-P
  FC

1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P17612 Human | P05132 Mouse | P27791 Rat
Alternative names: cAMP dependent protein kinase beta catalytic subunit cAMP dependent protein kinase alpha catalytic subunit cAMP dependent protein kinase catalytic subunit alpha cAMP dependent protein kinase catalytic subunit beta PKA C alpha PKA C beta PKACA PKACB PRKACA PRKACB Protein kinase cAMP dependent catalytic alpha Protein kinase cAMP dependent catalytic beta
Images
ER1706-65_1.jpg Fig1: ICC staining PKA C-alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-65_2.jpg Fig2: ICC staining PKA C-alpha in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-65_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PKA C-alpha antibody. Counter stained with hematoxylin.
ER1706-65_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKA C-alpha antibody. Counter stained with hematoxylin.
ER1706-65_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKA C-alpha antibody. Counter stained with hematoxylin.
ER1706-65_6.jpg Fig6: Flow cytometric analysis of PC-3M cells with PKA C-alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.