Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | IF-Cell, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 40.5 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human PKA C alpha aa 172-338. |
Positive control: | A431, PC-3M, rat brain tissue, human kidney tissue, mouse testis tissue. |
Subcellular location: | Mitochondrion. Nucleus.Plasma membrane. |
Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: P17612 Human | P05132 Mouse | P27791 Rat |
Alternative names: | cAMP dependent protein kinase beta catalytic subunit cAMP dependent protein kinase alpha catalytic subunit cAMP dependent protein kinase catalytic subunit alpha cAMP dependent protein kinase catalytic subunit beta PKA C alpha PKA C beta PKACA PKACB PRKACA PRKACB Protein kinase cAMP dependent catalytic alpha Protein kinase cAMP dependent catalytic beta |
Fig1: ICC staining PKA C-alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig2: ICC staining PKA C-alpha in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PKA C-alpha antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PKA C-alpha antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKA C-alpha antibody. Counter stained with hematoxylin. | |
Fig6: Flow cytometric analysis of PC-3M cells with PKA C-alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |