CD34 Rabbit Polyclonal Antibody
cat.: ER1706-66
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human CD34 aa 10-310/385. |
| Positive control: | TF-1 cell lysate, mouse lung tissue lysate, human liver cancer tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IHC-P |
1:500-2,000 1:100-1:500 |
| Uniprot #: | SwissProt: P28906 Human | Q64314 Mouse |
| Alternative names: | CD34 CD34 antigen CD34 molecule CD34_HUMAN Cluster designation 34 Hematopoietic progenitor cell antigen CD34 HPCA1 Mucosialin OTTHUMP00000034733 OTTHUMP00000034734 |
Images
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Fig1:
Western blot analysis of CD34 on different lysates with Rabbit anti-CD34 antibody (ER1706-66) at 1/2,000 dilution. Lane 1: TF-1 (Human erythroleukemia cell) cell lysate (15 µg/Lane) Lane 2: Mouse lung tissue lysate (30 µg/Lane) Exposure time: 110 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1706-66, 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 41 kDa Observed band size: 100 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CD34 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.