| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Zebrafish |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within zebrafish IL-7R alpha aa 202-372 / 372. |
| Positive control: | Zebrafish, Daudi. |
| Subcellular location: | Membrane. Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:5,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P16871 Human | E9QDP8 Zebrafish |
| Alternative names: | CD 127 CD127 CD127 antigen CDw127 IL 7R alpha IL 7R IL-7 receptor subunit alpha IL-7R subunit alpha IL-7R-alpha IL-7RA IL7R IL7RA IL7RA_HUMAN IL7Ralpha ILRA Interleukin 7 receptor alpha chain Interleukin 7 receptor Interleukin 7 receptor isoform H5 6 Interleukin-7 receptor subunit alpha |
|
Fig1:
Western blot analysis of IL-7R alpha on zebrafish tissue lysate with Rabbit anti-IL-7R alpha antibody (ER1706-68) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 180 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1706-68, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 52 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-IL-7R-alpha antibody. Counter stained with hematoxylin. |