IL-7R alpha Rabbit Polyclonal Antibody
cat.: ER1706-68
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Zebrafish
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 52 kDa
Isotype: IgG
Immunogen: Recombinant protein within zebrafish IL-7R alpha aa 202-372 / 372.
Positive control: Zebrafish, Daudi.
Subcellular location: Membrane. Secreted.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:5,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P16871 Human | E9QDP8 Zebrafish
Alternative names: CD 127 CD127 CD127 antigen CDw127 IL 7R alpha IL 7R IL-7 receptor subunit alpha IL-7R subunit alpha IL-7R-alpha IL-7RA IL7R IL7RA IL7RA_HUMAN IL7Ralpha ILRA Interleukin 7 receptor alpha chain Interleukin 7 receptor Interleukin 7 receptor isoform H5 6 Interleukin-7 receptor subunit alpha
Images
ER1706-68_1.jpg Fig1: Western blot analysis of IL-7R alpha on zebrafish tissue lysate with Rabbit anti-IL-7R alpha antibody (ER1706-68) at 1/5,000 dilution.

Lysates/proteins at 10 µg/Lane.
Exposure time: 180 seconds; ECL: K1802

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: ER1706-68, 1/5,000 in 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 52 kDa
ER1706-68_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded zebrafish tissue using anti-IL-7R-alpha antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.