Cullin-3 Rabbit Polyclonal Antibody
cat.: ER1706-70
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 89 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Cullin-3 aa 724-768. |
| Positive control: | SH-SY5Y, 293T, mouse testis tissue lysate, human kidney tissue, human placenta tissue. |
| Subcellular location: | Golgi apparatus. Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q13618 Human | Q9JLV5 Mouse |
| Alternative names: | CUL 3 Cul-3 cul3 CUL3_HUMAN Cullin-3 Cullin3 KIAA0617 PHA2E |
Images
|
Fig1:
Western blot analysis of Cullin-3 on 293T lysates with Rabbit anti-Cullin-3 antibody (ER1706-70) at 1/1,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1706-70, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 89 kDa Observed band size: 89 kDa |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Cullin-3 antibody (ER1706-70) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-70) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin. |
|
Fig4:
Western blot analysis of Cullin-3 on mouse testis tissue lysates with Rabbit anti-Cullin-3 antibody (ER1706-70) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 89 kDa Observed band size: 80 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-70) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.