BMAL1 Rabbit Polyclonal Antibody
cat.: ER1706-71
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N-terminal human BMAL1. |
| Positive control: | SH-SY5Y cell lysates, SH-SY5Y, human liver cncer tissue, human prostate tissue, mouse brain tissue, mouse prostate tissue, rat brain tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-2,000 1:100-1:500 1:50-1:200 |
| Uniprot #: | SwissProt: O00327 Human | Q9WTL8 Mouse | Q9EPW1 Rat |
| Alternative names: | ARNT like protein 1 brain and muscle Arntl Aryl hydrocarbon receptor nuclear translocator like Aryl hydrocarbon receptor nuclear translocator like protein 1 Aryl hydrocarbon receptor nuclear translocator-like protein 1 Basic helix loop helix PAS orphan MOP3 Basic helix loop helix PAS protein MOP3 Basic-helix-loop-helix-PAS protein MOP3 bHLH PAS protein JAP3 bHLH-PAS protein JAP3 bHLHe5 BMAL 1 BMAL1_HUMAN BMAL1c Brain and muscle ARNT like 1 Brain and muscle ARNT-like 1 CG8727 PA Class E basic helix-loop-helix protein 5 cycle JAP 3 JAP3 Member of PAS protein 3 Member of PAS superfamily 3 MGC47515 MOP 3 MOP3 PAS domain-containing protein 3 PASD 3 PASD3 TIC |
Images
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Fig1: Western blot analysis of BMAL1 on SH-SY5Y cell lysates using anti-BMAL1 antibody at 1/1,000 dilution. |
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Fig2:
Immunocytochemistry analysis of SH-SY5Y cells labeling BMAL1 with Rabbit anti-BMAL1 antibody (ER1706-71) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-BMAL1 antibody (ER1706-71) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver cncer tissue using anti-BMAL1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-BMAL1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-BMAL1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-BMAL1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-BMAL1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.