BMAL1 Rabbit Polyclonal Antibody
cat.: ER1706-71
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 69 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N-terminal human BMAL1. |
| Positive control: | SYSH5Y cell lysates, A549, MCF-7, human liver cncer tissue, human prostate tissue, mouse brain tissue, mouse prostate tissue, rat brain tissue. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-2,000 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O00327 Human | Q9WTL8 Mouse | Q9EPW1 Rat |
| Alternative names: | ARNT like protein 1 brain and muscle Arntl Aryl hydrocarbon receptor nuclear translocator like Aryl hydrocarbon receptor nuclear translocator like protein 1 Aryl hydrocarbon receptor nuclear translocator-like protein 1 Basic helix loop helix PAS orphan MOP3 Basic helix loop helix PAS protein MOP3 Basic-helix-loop-helix-PAS protein MOP3 bHLH PAS protein JAP3 bHLH-PAS protein JAP3 bHLHe5 BMAL 1 BMAL1_HUMAN BMAL1c Brain and muscle ARNT like 1 Brain and muscle ARNT-like 1 CG8727 PA Class E basic helix-loop-helix protein 5 cycle JAP 3 JAP3 Member of PAS protein 3 Member of PAS superfamily 3 MGC47515 MOP 3 MOP3 PAS domain-containing protein 3 PASD 3 PASD3 TIC |
Images
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Fig1: Western blot analysis of BMAL1 on SYSH5Y cell lysates using anti-BMAL1 antibody at 1/1,000 dilution. |
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Fig2: ICC staining BMAL1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining BMAL1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver cncer tissue using anti-BMAL1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-BMAL1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-BMAL1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-BMAL1 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-BMAL1 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of SHSY5Y cells with BMAL1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.