NLRP3 Rabbit Polyclonal Antibody
cat.: ER1706-72
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 85 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human NLRP3 aa 987-1,036 / 1,036.
Positive control: LOVO cell lysate, A431, HepG2, LOVO, human liver cancer tissue, human colon cancer tissue, human placenta tissue, mouse brain tissue, mouse kidney tissue, Jurkat.
Subcellular location: Nucleus. Cytoplasm. Secreted.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:2,000
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q96P20 Human | Q8R4B8 Mouse
Alternative names: AGTAVPRL AII/AVP Angiotensin/vasopressin receptor AII/AVP like Angiotensin/vasopressin receptor AII/AVP-like C1orf7 Caterpiller protein 1.1 CIAS 1 CIAS1 CLR1.1 Cold autoinflammatory syndrome 1 Cold autoinflammatory syndrome 1 protein Cryopyrin Familial cold autoinflammatory syndrome FCAS FCU LRR and PYD domains-containing protein 3 Muckle-Wells syndrome MWS NACHT NACHT LRR and PYD containing protein 3 NALP 3 NALP3 NALP3_HUMAN NLR family pyrin domain containing 3 NLRP3 PYPAF 1 PYPAF1 PYRIN containing APAF1 like protein 1 PYRIN-containing APAF1-like protein 1
Images
ER1706-72_1.jpg Fig1: Western blot analysis of NLRP3 on LOVO cell lysates with Rabbit anti-NLRP3 antibody (ER1706-72) at 1/500 dilution.

Lysates/proteins at 30 µg/Lane.

Predicted band size: 118 kDa
Observed band size: 118 kDa

Exposure time: 1 minute;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-72) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER1706-72_2.jpg Fig2: ICC staining NLRP3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-72_3.jpg Fig3: ICC staining NLRP3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-72_4.jpg Fig4: ICC staining NLRP3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-72_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
ER1706-72_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
ER1706-72_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
ER1706-72_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
ER1706-72_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
ER1706-72_10.jpg Fig10: Flow cytometric analysis of Jurkat cells with NLRP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.