NLRP3 Rabbit Polyclonal Antibody
cat.: ER1706-72
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 118 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human NLRP3 aa 987-1,036 / 1,036. |
| Positive control: | LOVO cell lysate, A431, HepG2, LOVO, human liver cancer tissue, human colon cancer tissue, human placenta tissue, mouse brain tissue, mouse kidney tissue, Jurkat. |
| Subcellular location: | Nucleus. Cytoplasm. Secreted. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2,000 1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q96P20 Human | Q8R4B8 Mouse |
| Alternative names: | AGTAVPRL AII/AVP Angiotensin/vasopressin receptor AII/AVP like Angiotensin/vasopressin receptor AII/AVP-like C1orf7 Caterpiller protein 1.1 CIAS 1 CIAS1 CLR1.1 Cold autoinflammatory syndrome 1 Cold autoinflammatory syndrome 1 protein Cryopyrin Familial cold autoinflammatory syndrome FCAS FCU LRR and PYD domains-containing protein 3 Muckle-Wells syndrome MWS NACHT NACHT LRR and PYD containing protein 3 NALP 3 NALP3 NALP3_HUMAN NLR family pyrin domain containing 3 NLRP3 PYPAF 1 PYPAF1 PYRIN containing APAF1 like protein 1 PYRIN-containing APAF1-like protein 1 |
Images
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Fig1:
Western blot analysis of NLRP3 on LOVO cell lysates with Rabbit anti-NLRP3 antibody (ER1706-72) at 1/500 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 118 kDa Observed band size: 118 kDa Exposure time: 1 minute; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-72) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining NLRP3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining NLRP3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining NLRP3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NLRP3 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NLRP3 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NLRP3 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NLRP3 antibody. Counter stained with hematoxylin. |
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Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NLRP3 antibody. Counter stained with hematoxylin. |
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Fig10: Flow cytometric analysis of Jurkat cells with NLRP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.