Fyn Rabbit Polyclonal Antibody
cat.: ER1706-74
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within N-terminal human Fyn. |
| Positive control: | Mouse brain tissue lysate, Rat brain tissue lysate. |
| Subcellular location: | Cytoplasm. Membrane. Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:10,000 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: P06241 Human | P39688 Mouse | Q62844 Rat |
| Alternative names: | C syn protooncogene Fyn FYN oncogene related to SRC FGR YES FYN_HUMAN OKT3 induced calcium influx regulator P59 FYN p59-Fyn Protein tyrosine kinase fyn Proto oncogene tyrosine protein kinase fyn Proto-oncogene c-Fyn Proto-oncogene Syn Protooncogene Syn SLK Src like kinase Src yes related novel gene Src-like kinase Src/yes related novel SYN Tyrosine kinase p59fyn T Tyrosine kinase p59fyn(T) Tyrosine-protein kinase Fyn |
Images
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Fig1:
Western blot analysis of Fyn on different lysates with Rabbit anti-Fyn antibody (ER1706-74) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1706-74, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 61 kDa Observed band size: 61 kDa |
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Fig2: ICC staining Fyn in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Fyn in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Fyn in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.