ATP citrate lyase Rabbit Polyclonal Antibody
cat.: ER1706-75
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 121 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human ATP citrate lyase aa 813-1066. |
| Positive control: | HeLa cell lysate, NIH-3T3 cell lysate, A549, SH-SY5Y, rat brain tissue, human colon cancer tissue, human pancreas tissue, mouse testis tissue. |
| Subcellular location: | Cytoplasm, cytosol. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:5000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P53396 Human | Q91V92 Mouse | P16638 Rat |
| Alternative names: | ACL Acly ACLY_HUMAN ATP citrate (pro-S) lyase ATP citrate lyase ATP citrate synthase ATP-citrate (pro-S-)-lyase ATP-citrate synthase ATPcitrate synthase ATPCL Citrate cleavage enzyme CLATP OTTHUMP00000164773 |
Images
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Fig1:
Western blot analysis of ATP citrate lyase on different lysates with Rabbit anti-ATP citrate lyase antibody (ER1706-75) at 1/5,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 20 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1706-75, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 120.8 kDa Observed band size: 121 kDa |
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Fig2:
All lanes: Western blot analysis of ATP citrate lyase with anti-ATP citrate lyase antibody (ER1706-75) at 1:500 dilution. Lane 1: Wild-type A549 whole cell lysate (10 µg). Lane 2: ATP citrate lyase knockout A549 whole cell lysate (10 µg). ER1706-75 was shown to specifically react with ATP citrate lyase in wild-type A549 cells. No band was observed when ATP citrate lyase knockout sample was tested. Wild-type and ATP citrate lyase knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER1706-75, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig3: ICC staining ATP citrate lyase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining ATP citrate lyase in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.