Anti-ATP citrate lyase antibody
cat.: ER1706-75
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 122 kDa
Isotype: IgG
Immunogen: Recombinant protein within human ATP citrate lyase aa 813-1066.
Positive control: NIH-3T3, A549, SH-SY5Y, mouse pancreas tissue lysate, rat brain tissue, human colon cancer tissue, human pancreas tissue, mouse testis tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P53396 Human | Q91V92 Mouse | P16638 Rat
Alternative names: ACL Acly ACLY_HUMAN ATP citrate (pro-S) lyase ATP citrate lyase ATP citrate synthase ATP-citrate (pro-S-)-lyase ATP-citrate synthase ATPcitrate synthase ATPCL Citrate cleavage enzyme CLATP OTTHUMP00000164773
Images
ER1706-75_1.jpg Fig1: Western blot analysis of ATP citrate lyase on mouse pancreas tissue (1) and NIH-3T3 cell (2) lysate using anti-ATP citrate lyase antibody at 1/200 dilution.
ER1706-75_2.jpg Fig2: ICC staining ATP citrate lyase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-75_3.jpg Fig3: ICC staining ATP citrate lyase in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-75_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
ER1706-75_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
ER1706-75_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
ER1706-75_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
ER1706-75_8.jpg Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.