ATP citrate lyase Rabbit Polyclonal Antibody
cat.: ER1706-75
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 121 kDa
Isotype: IgG
Immunogen: Recombinant protein within human ATP citrate lyase aa 813-1066.
Positive control: NIH-3T3, A549, SH-SY5Y, mouse pancreas tissue lysate, rat brain tissue, human colon cancer tissue, human pancreas tissue, mouse testis tissue.
Subcellular location: Cytoplasm, cytosol.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P53396 Human | Q91V92 Mouse | P16638 Rat
Alternative names: ACL Acly ACLY_HUMAN ATP citrate (pro-S) lyase ATP citrate lyase ATP citrate synthase ATP-citrate (pro-S-)-lyase ATP-citrate synthase ATPcitrate synthase ATPCL Citrate cleavage enzyme CLATP OTTHUMP00000164773
Images
ER1706-75_1.jpg Fig1: Western blot analysis of ATP citrate lyase on mouse pancreas tissue (1) and NIH-3T3 cell (2) lysate using anti-ATP citrate lyase antibody at 1/200 dilution.
ER1706-75_2.jpg Fig2: All lanes: Western blot analysis of ATP citrate lyase with anti-ATP citrate lyase antibody (ER1706-75) at 1:500 dilution.
Lane 1: Wild-type A549 whole cell lysate (10 µg).
Lane 2: ATP citrate lyase knockout A549 whole cell lysate (10 µg).

ER1706-75 was shown to specifically react with ATP citrate lyase in wild-type A549 cells. No band was observed when ATP citrate lyase knockout sample was tested. Wild-type and ATP citrate lyase knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER1706-75, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER1706-75_3.jpg Fig3: ICC staining ATP citrate lyase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-75_4.jpg Fig4: ICC staining ATP citrate lyase in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-75_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
ER1706-75_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
ER1706-75_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
ER1706-75_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.
ER1706-75_9.jpg Fig9: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.