Anti-MMP-3 antibody
cat.: ER1706-77
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 54 kDa
Isotype: IgG
Immunogen: Recombinant protein within C-terminal human MMP-3.
Positive control: A549, LOVO, rat brain tissue, human tonsil tissue, human liver tissue, human colon cancer tissue, mouse brain tissue.
Subcellular location: Extracellular matrix. Secreted.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P08254 Human | P28862 Mouse | P03957 Rat
Alternative names: CHDS6 Matrix metalloproteinase 3 Matrix metalloproteinase-3 MGC126102 MGC126103 MGC126104 MMP 3 MMP-3 MMP3 MMP3_HUMAN Proteoglycanase SL-1 SL1 STMY STMY1 STR1 Stromelysin 1 Stromelysin-1 Transin 1 Transin-1
Images
ER1706-77_1.jpg Fig1: Western blot analysis of MMP-3 on LOVO cell lysate using anti-MMP-3 antibody at 1/200 dilution.
ER1706-77_2.jpg Fig2: ICC staining MMP-3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-77_3.jpg Fig3: ICC staining MMP-3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-77_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
ER1706-77_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
ER1706-77_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
ER1706-77_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
ER1706-77_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
ER1706-77_9.jpg Fig9: Flow cytometric analysis of LOVO cells with MMP-3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.