MMP-3 Rabbit Polyclonal Antibody
cat.: ER1706-77
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 54 kDa
Isotype: IgG
Immunogen: Recombinant protein within C-terminal human MMP-3.
Positive control: Hela cell lysate, Jurkat cell lysate, U87MG cell lysate, mouse liver tissue lysate, mouse brain tissue lysate, mouse skeletal muscle tissue lysate, rat kidney tissue lysate, A549, LOVO, rat brain tissue, human tonsil tissue, human liver tissue, human colon cancer tissue, mouse brain tissue.
Subcellular location: Extracellular matrix. Secreted.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P08254 Human | P28862 Mouse | P03957 Rat
Alternative names: CHDS6 Matrix metalloproteinase 3 Matrix metalloproteinase-3 MGC126102 MGC126103 MGC126104 MMP 3 MMP-3 MMP3 MMP3_HUMAN Proteoglycanase SL-1 SL1 STMY STMY1 STR1 Stromelysin 1 Stromelysin-1 Transin 1 Transin-1
Images
ER1706-77_1.jpg Fig1: Western blot analysis of MMP-3 on different lysates with Rabbit anti-MMP-3 antibody (ER1706-77) at 1/500 dilution.

Lane 1: Hela cell lysate (10 µg/Lane)
Lane 2: Jurkat cell lysate (10 µg/Lane)
Lane 3: U87MG cell lysate (10 µg/Lane)
Lane 4: Mouse liver tissue lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (20 µg/Lane)
Lane 6: Mouse skeletal muscle tissue lysate (20 µg/Lane)
Lane 7: Rat kidney tissue lysate (20 µg/Lane)

Predicted band size: 54 kDa
Observed band size: 45 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-77) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER1706-77_2.jpg Fig2: ICC staining MMP-3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-77_3.jpg Fig3: ICC staining MMP-3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-77_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
ER1706-77_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
ER1706-77_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
ER1706-77_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
ER1706-77_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
ER1706-77_9.jpg Fig9: Flow cytometric analysis of LOVO cells with MMP-3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.