ER1706-77

MMP-3 Rabbit Polyclonal Antibody

cat.: ER1706-77

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P, FC
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 54 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within C-terminal human MMP-3.
Positive control:	U-87 MG cell lysate, HeLa cell lysate, A549, LOVO, human liver tissue, mouse liver tissue, rat liver tissue, rat brain tissue, human tonsil tissue, human colon cancer tissue, mouse brain tissue.
Subcellular location:	Extracellular matrix. Secreted.
Recommended Dilutions: WB IF-Cell IHC-P FC	1:1,000 1:50-1:200 1:1,000 1:50-1:100
Uniprot #:	SwissProt: P08254 Human \| P28862 Mouse \| P03957 Rat
Alternative names:	CHDS6 Matrix metalloproteinase 3 Matrix metalloproteinase-3 MGC126102 MGC126103 MGC126104 MMP 3 MMP-3 MMP3 MMP3_HUMAN Proteoglycanase SL-1 SL1 STMY STMY1 STR1 Stromelysin 1 Stromelysin-1 Transin 1 Transin-1

Images

	Fig1: Western blot analysis of MMP-3 on different lysates with Rabbit anti-MMP-3 antibody (ER1706-77) at 1/1,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: HeLa cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 59 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-77) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
	Fig2: ICC staining MMP-3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining MMP-3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

	Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-MMP-3 antibody (ER1706-77) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1706-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-MMP-3 antibody (ER1706-77) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1706-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-MMP-3 antibody (ER1706-77) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1706-77) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig7: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
	Fig8: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
	Fig9: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.
	Fig10: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MMP-3 antibody. Counter stained with hematoxylin.

Fig11: Flow cytometric analysis of LOVO cells with MMP-3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.