FBXW7 Rabbit Polyclonal Antibody
cat.: ER1706-78
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, FC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 70 kDa
Isotype: IgG
Immunogen: Recombinant protein within human FBXW7 aa 84-327.
Positive control: Mouse spleen tissue lysate, rat lung tissue lysate, HepG2 cell lysate, HUVEC, SH-SY5Y.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  ICC
  FC
  IHC-P

1:500-1:1,000
1:500-1:2,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: Q969H0 Human | Q8VBV4 Mouse | D3Z902 Rat
Alternative names: AGO Archipelago Archipelago homolog Archipelago, Drosophila, homolog of CDC4 CDC4, S.cerevisiae, homolog of DKFZp686F23254 F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) F box and WD 40 domain protein 7 F box and WD repeat domain containing 7 F box protein FBW7 F box protein FBX30 F box protein SEL 10 F box protein SEL10 F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase F-box and WD-40 domain-containing protein 7 F-box protein FBX30 F-box/WD repeat-containing protein 7 FBW6 FBW7 FBX30 FBXO30 FBXW6 FBXW7 FBXW7_HUMAN FLJ16457 hAgo hCdc4 Homolog of C elegans sel 10 Homolog of C.elegans sel10 SEL 10 SEL-10 SEL10
Images
ER1706-78_1.jpg Fig1: Western blot analysis of FBXW7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1706-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse spleen tissue lysate
Lane 1: Rat lung tissue lysate
Lane 2: HepG2 cell lysate
ER1706-78_2.jpg Fig2: ICC staining of FBXW7 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1706-78, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1706-78_3.jpg Fig3: ICC staining of FBXW7 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1706-78, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1706-78_4.jpg Fig4: Flow cytometric analysis of FBXW7 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1706-78, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.