Fbxw7 Rabbit Polyclonal Antibody
cat.: ER1706-78
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, FC, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 80 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Fbxw7aa 84-327.
Positive control: Mouse spleen tissue lysate, rat lung tissue lysate, HepG2 cell lysate, HUVEC, SH-SY5Y, human colon tissue, mouse colon tissue, rat colon tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  FC
  IHC-P
1:500-1:1,000
1:500-1:2,000
1:50-1:100
1:2,000
Uniprot #: SwissProt: Q969H0 Human | Q8VBV4 Mouse | D3Z902 Rat
Alternative names: AGO Archipelago Archipelago homolog Archipelago, Drosophila, homolog of CDC4 CDC4, S.cerevisiae, homolog of DKFZp686F23254 F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) F box and WD 40 domain protein 7 F box and WD repeat domain containing 7 F box protein FBW7 F box protein FBX30 F box protein SEL 10 F box protein SEL10 F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase F-box and WD-40 domain-containing protein 7 F-box protein FBX30 F-box/WD repeat-containing protein 7 FBW6 FBW7 FBX30 FBXO30 FBXW6 FBXW7 FBXW7_HUMAN FLJ16457 hAgo hCdc4 Homolog of C elegans sel 10 Homolog of C.elegans sel10 SEL 10 SEL-10 SEL10
Images
ER1706-78_1.jpg Fig1: Western blot analysis of Fbxw7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1706-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse spleen tissue lysate
Lane 2: Rat lung tissue lysate
Lane 3: HepG2 cell lysate
ER1706-78_2.jpg Fig2: ICC staining of Fbxw7 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1706-78, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1706-78_3.jpg Fig3: ICC staining of Fbxw7 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1706-78, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1706-78_4.jpg Fig4: Flow cytometric analysis of Fbxw7 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1706-78, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
ER1706-78_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Fbxw7 antibody (ER1706-78) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-78) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1706-78_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Fbxw7 antibody (ER1706-78) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-78) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1706-78_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-Fbxw7 antibody (ER1706-78) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1706-78) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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