FBXW7 Rabbit Polyclonal Antibody
cat.: ER1706-78
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 80 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human FBXW7 aa 84-327. |
| Positive control: | Mouse spleen tissue lysate, rat lung tissue lysate, HepG2 cell lysate, HUVEC, SH-SY5Y. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC IHC-P |
1:500-1:1,000 1:500-1:2,000 1:50-1:100 1:50-1:200 |
| Uniprot #: | SwissProt: Q969H0 Human | Q8VBV4 Mouse | D3Z902 Rat |
| Alternative names: | AGO Archipelago Archipelago homolog Archipelago, Drosophila, homolog of CDC4 CDC4, S.cerevisiae, homolog of DKFZp686F23254 F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) F box and WD 40 domain protein 7 F box and WD repeat domain containing 7 F box protein FBW7 F box protein FBX30 F box protein SEL 10 F box protein SEL10 F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase F-box and WD-40 domain-containing protein 7 F-box protein FBX30 F-box/WD repeat-containing protein 7 FBW6 FBW7 FBX30 FBXO30 FBXW6 FBXW7 FBXW7_HUMAN FLJ16457 hAgo hCdc4 Homolog of C elegans sel 10 Homolog of C.elegans sel10 SEL 10 SEL-10 SEL10 |
Images
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Fig1:
Western blot analysis of FBXW7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1706-78, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse spleen tissue lysate Lane 2: Rat lung tissue lysate Lane 3: HepG2 cell lysate |
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Fig2: ICC staining of FBXW7 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1706-78, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining of FBXW7 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1706-78, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Flow cytometric analysis of FBXW7 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1706-78, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.