FOXO3A Rabbit Polyclonal Antibody
cat.: ER1706-79
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 71 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal human FOXO3A.
Positive control: SH-SY5Y, K562, A431, LOVO, human tonsil tissue, mouse testis tissue, mouse fallopian tissue, SHSY5Y.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O43524 Human | Q9WVH4 Mouse
Alternative names: AF6q21 AF6q21 protein DKFZp781A0677 FKHR2 FKHRL 1 FKHRL1 FKHRL1P2 Forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1 Forkhead box O3 Forkhead box O3A Forkhead box protein O3 Forkhead box protein O3A Forkhead Drosophila homolog of in rhabdomyosarcoma like 1 Forkhead homolog (rhabdomyosarcoma) like 1 Forkhead in rhabdomyosarcoma like 1 Forkhead in rhabdomyosarcoma-like 1 FOX O3A FOXO2 foxo3 FOXO3_HUMAN FOXO3A MGC12739 MGC31925
Images
ER1706-79_1.jpg Fig1: Western blot analysis of FOXO3A on different cell lysate using anti-FOXO3A antibody at 1/500 dilution.
Positive control:
Lane 1: SH-SY5Y
Lane 2: K562
ER1706-79_2.jpg Fig2: ICC staining FOXO3A in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-79_3.jpg Fig3: ICC staining FOXO3A in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-79_4.jpg Fig4: ICC staining FOXO3A in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-79_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-FOXO3A antibody. Counter stained with hematoxylin.
ER1706-79_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-FOXO3A antibody. Counter stained with hematoxylin.
ER1706-79_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-FOXO3A antibody. Counter stained with hematoxylin.
ER1706-79_8.jpg Fig8: Flow cytometric analysis of SHSY5Y cells with FOXO3A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.