CD4 Rabbit Polyclonal Antibody
cat.: ER1706-80
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human CD4 aa 45-109.
Positive control: Hela, HepG2, SH-SY5Y, Jurkat, human tonsil tissue, human spleen tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P01730 Human
Alternative names: CD 4 CD4 (L3T4) CD4 CD4 antigen (p55) CD4 antigen CD4 molecule CD4 receptor CD4+ Lymphocyte deficiency, included CD4_HUMAN CD4mut L3T4 Leu3 Ly-4 Lymphocyte antigen CD4 MGC165891 OTTHUMP00000238897 p55 T cell antigen T4 T cell antigen T4/LEU3 T cell differentiation antigen L3T4 T cell OKT4 deficiency, included T cell surface antigen T4/Leu 3 T cell surface antigen T4/Leu3 T cell surface glycoprotein CD4 T-cell surface antigen T4/Leu-3 T-cell surface glycoprotein CD4 W3/25 W3/25 antigen
Images
ER1706-80_1.jpg Fig1: ICC staining CD4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-80_2.jpg Fig2: ICC staining CD4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-80_3.jpg Fig3: ICC staining CD4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-80_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD4 antibody. Counter stained with hematoxylin.
ER1706-80_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD4 antibody. Counter stained with hematoxylin.
ER1706-80_6.jpg Fig6: Flow cytometric analysis of Jurkat cells with CD4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.