Anti-CD4 antibody
cat.: ER1706-80
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 51 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human CD4 aa 45-109.
Positive control: Mouse spleen tissue lysate, mouse thymus tissue lysate, Hela, HepG2, SH-SY5Y, Jurkat, human tonsil tissue, human spleen tissue, mouse colon tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P01730 Human | P06332 Mouse
Alternative names: CD 4 antibody CD4 (L3T4) antibody CD4 antibody CD4 antigen (p55) antibody CD4 antigen antibody CD4 molecule antibody CD4 receptor antibody CD4+ Lymphocyte deficiency, included antibody CD4_HUMAN antibody CD4mut antibody L3T4 antibody Leu3 antibody Ly-4 antibody Lymphocyte antigen CD4 antibody MGC165891 antibody OTTHUMP00000238897 antibody p55 antibody T cell antigen T4 antibody T cell antigen T4/LEU3 antibody T cell differentiation antigen L3T4 antibody T cell OKT4 deficiency, included antibody T cell surface antigen T4/Leu 3 antibody T cell surface antigen T4/Leu3 antibody T cell surface glycoprotein CD4 antibody T-cell surface antigen T4/Leu-3 antibody T-cell surface glycoprotein CD4 antibody W3/25 antibody W3/25 antigen antibody
Images
ER1706-80_1.jpg Fig1: Western blot analysis of CD4 on different tissue lysates using anti-CD4 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse spleen
Lane 2: Mouse thymus
ER1706-80_2.jpg Fig2: ICC staining CD4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-80_3.jpg Fig3: ICC staining CD4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-80_4.jpg Fig4: ICC staining CD4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-80_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD4 antibody. Counter stained with hematoxylin.
ER1706-80_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD4 antibody. Counter stained with hematoxylin.
ER1706-80_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-CD4 antibody. Counter stained with hematoxylin.
ER1706-80_8.jpg Fig8: Flow cytometric analysis of Jurkat cells with CD4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.