RAR alpha Rabbit Polyclonal Antibody
cat.: ER1706-81
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Recombinant protein within human RAR alpha aa 135-406.
Positive control: SK-Br-3 cell lysate, Hela cell lysate, A431, MCF-7, SH-SY5Y, rat brain tissue, mouse testis tissue, mouse brain tissue.
Subcellular location: Nucleus. Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500
1:500-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P10276 Human | P11416 Mouse
Entrez Gene: 24705 Rat
Alternative names: NR1B1 Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein Nuclear receptor subfamily 1 group B member 1 Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form RAR alpha RAR RAR-alpha rara RARA_HUMAN RARalpha RARalpha1 Retinoic acid nuclear receptor alpha variant 1 Retinoic acid nuclear receptor alpha variant 2 Retinoic acid receptor alpha Retinoic acid receptor alpha polypeptide
Images
ER1706-81_1.jpg Fig1: Western blot analysis of RAR alpha on different lysates with Rabbit anti-RAR alpha antibody (ER1706-81) at 1/500 dilution.

Lane 1: SK-Br-3 cell lysate
Lane 2: Hela cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 92 kDa
Observed band size: 92 kDa

Exposure time: 2 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-81) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER1706-81_2.jpg Fig2: ICC staining RAR alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-81_3.jpg Fig3: ICC staining RAR alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-81_4.jpg Fig4: ICC staining RAR alpha in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-81_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RAR alpha antibody. Counter stained with hematoxylin.
ER1706-81_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RAR alpha antibody. Counter stained with hematoxylin.
ER1706-81_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse brian tissue using anti-RAR alpha antibody. Counter stained with hematoxylin.
ER1706-81_8.jpg Fig8: Flow cytometric analysis of MCF-7 cells with RAR alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.