RAR alpha Rabbit Polyclonal Antibody
cat.: ER1706-81
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human RAR alpha aa 135-406. |
| Positive control: | SK-Br-3 cell lysate, Hela cell lysate, A431, MCF-7, SH-SY5Y, rat brain tissue, mouse testis tissue, mouse brain tissue. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P10276 Human | P11416 Mouse Entrez Gene: 24705 Rat |
| Alternative names: | NR1B1 Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein Nuclear receptor subfamily 1 group B member 1 Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form RAR alpha RAR RAR-alpha rara RARA_HUMAN RARalpha RARalpha1 Retinoic acid nuclear receptor alpha variant 1 Retinoic acid nuclear receptor alpha variant 2 Retinoic acid receptor alpha Retinoic acid receptor alpha polypeptide |
Images
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Fig1:
Western blot analysis of RAR alpha on different lysates with Rabbit anti-RAR alpha antibody (ER1706-81) at 1/500 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: Hela cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 51 kDa Observed band size: 51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-81) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining RAR alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining RAR alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining RAR alpha in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RAR alpha antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RAR alpha antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse brian tissue using anti-RAR alpha antibody. Counter stained with hematoxylin. |
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Fig8: Flow cytometric analysis of MCF-7 cells with RAR alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.