Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 77 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human ZBTB48 aa 584-633 / 688. |
Positive control: | Mouse spleen tissue lysate, HepG2, PC-3M, SH-SY5Y, human liver cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue. |
Subcellular location: | Nucleus. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: P10074 Human | Q1H9T6 Mouse Entrez Gene: 362668 Rat |
Alternative names: | 0610011D15Rik AI327031 GLI Kruppel family member HKR3 HKR3 Krueppel related zinc finger protein 3 Krueppel-related zinc finger protein 3 OTTMUSP00000031803 pp9964 Protein HKR3 RP23-445E20.3 ZBT48_HUMAN ZBTB 48 Zbtb48 Zinc finger and BTB domain containing 48 Zinc finger and BTB domain containing protein 48 Zinc finger and BTB domain-containing protein 48 Zinc finger protein 855 ZNF855 |
Fig1:
Western blot analysis of ZBTB48 on mouse spleen tissue lysate using anti-ZBTB48 antibody at 1/1,000 dilution. |
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Fig2: ICC staining ZBTB48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining ZBTB48 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin. | |
Fig7: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ZBTB48 antibody. Counter stained with hematoxylin. | |
Fig8: Flow cytometric analysis of Hela cells with ZBTB48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody. |