GAPDH Rabbit Polyclonal Antibody
cat.: ER1706-83
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human GAPDH aa 171-226. |
| Positive control: | PC-12, L929, NIH/3T3, F9, mouse liver tissue lysate, human tonsil tissue, human pancreas tissue, mouse testis tissue, MCF-7. |
| Subcellular location: | Cytoskeleton. Cytosol. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:2,000-5,000 1:50-1:200 1:50-1:200 1:50-1;100 |
| Uniprot #: | SwissProt: P04406 Human | P16858 Mouse | P04797 Rat |
| Alternative names: | 38 kDa BFA-dependent ADP-ribosylation substrate aging associated gene 9 protein Aging-associated gene 9 protein BARS-38 cb609 EC 1.2.1.12 Epididymis secretory sperm binding protein Li 162eP G3P_HUMAN G3PD G3PDH GAPD GAPDH Glyceraldehyde 3 phosphate dehydrogenase Glyceraldehyde-3-phosphate dehydrogenase HEL-S-162eP KNC-NDS6 MGC102544 MGC102546 MGC103190 MGC103191 MGC105239 MGC127711 MGC88685 OCAS, p38 component OCT1 coactivator in S phase, 38-KD component peptidyl cysteine S nitrosylase GAPDH Peptidyl-cysteine S-nitrosylase GAPDH wu:fb33a10 |
Images
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Fig1:
Western blot analysis of GAPDH on different lysates using anti-GAPDH antibody at 1/2,000 dilution. Positive control: Lane 1: PC-12 Lane 2: L929 Lane 3: NIH/3T3 Lane 4: F9 Lane 5: Mouse liver tissue |
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Fig2: ICC staining GAPDH in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GAPDH antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GAPDH antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GAPDH antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of MCF-7 cells with GAPDH antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.