FAP Rabbit Polyclonal Antibody
cat.: ER1706-84
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 95 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human FAP aa 711-760 / 760.
Positive control: Hela, NIH-3T3, Siha, SH-SY5Y.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  FC
1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q12884 Human | P97321 Mouse
Alternative names: Prolyl endopeptidase FAP 170 kDa melanoma membrane-bound gelatinase Dipeptidyl peptidase FAP Fibroblast activation protein alpha FAPalpha Gelatine degradation protease FAP Integral membrane serine protease Post-proline cleaving enzyme Serine integral membrane protease SIMP Surface-expressed protease Seprase FAP
Images
ER1706-84_1.jpg Fig1: Western blot analysis of FAP on Siha cell lysate using anti-FAP antibody at 1/1,000 dilution.
ER1706-84_2.jpg Fig2: ICC staining FAP in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-84_3.jpg Fig3: ICC staining FAP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
ER1706-84_4.jpg Fig4: Flow cytometric analysis of SH-SY5Y cells with FAP antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.