| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 46 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Estrogen Related Receptor alpha aa 206-416. |
| Positive control: | MCF-7 cell lysate, MCF-7. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB FC |
1:500 1:50-1:100 |
| Uniprot #: | SwissProt: P11474 Human |
| Alternative names: | Err 1 ERR a ERR alpha ERR-alpha Err1 ERR1 protein ERR1_HUMAN ERRa ERRalpha ESRL 1 ESRL1 ESRR A Esrra Estrogen receptor like 1 Estrogen receptor related 1 estrogen receptor related receptor alpha Estrogen receptor-like 1 Estrogen related receptor alpha Estrogen-related receptor alpha Estrra hERR1 NR3B1 Nuclear receptor subfamily 3 group B member 1 Steroid hormone receptor ERR1 |
|
Fig1:
Western blot analysis of Estrogen Related Receptor alpha on MCF-7 cell lysate with Rabbit anti-Estrogen Related Receptor alpha antibody (ER1706-85) at 1/500 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 30 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1706-85, 1/500 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 46 kDa Observed band size: 46 kDa |