TrkA Rabbit Polyclonal Antibody
cat.: ER1706-86
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 87 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TrkA aa 520-796 / 796. |
| Positive control: | SHSY5Y cell lysate, SHG-44 cell lysate, PC-12, Human liver cancer tissue, human stomach cancer tissue, mouse brain tissue, rat brain tissue. |
| Subcellular location: | Cell membrane, Endosome, Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:100 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P04629 Human | Q3UFB7 Mouse | P35739 Rat |
| Alternative names: | gp140trk High affinity nerve growth factor receptor High affinity nerve growth factor receptor precursor MTC Neurotrophic tyrosine kinase receptor type 1 NTRK1 NTRK1_HUMAN Oncogene TRK p14-TrkA p140 TrkA p140-TrkA Slow nerve growth Trk A TRK Trk-A TRK1 TRK1-transforming tyrosine kinase protein Tropomyosin-related kinase A Tyrosine kinase receptor A Tyrosine kinase receptor |
Images
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Fig1:
Western blot analysis of TrkA on SHSY5Y (1) and SHG-44 (2) cell lysates using anti-TrkA antibody at 1/200 dilution. |
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Fig2: ICC staining TrkA in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TrkA antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-TrkA antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TrkA antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TrkA antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of SHSY5Y cells with TrkA 1/2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.